Human Lactamase Beta (LACTb) RTU ELISA Kit | LACTb rtu elisa kit

Human Lactamase Beta (LACTb) ELISA Kit

Cat. Num. AAA4500671

• Gene Names: LACTB; G24; MRPL56
• Reactivity: Human
• Synonyms: Lactamase Beta (LACTb); Human Lactamase Beta (LACTb) ELISA Kit; G24; MRPL56; Mitochondrial Ribosomal Protein L56; Serine beta-lactamase-like protein LACTB; mitochondrial; LACTb rtu elisa kit

• Reactivity: Human
• Sequence Length: 332

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.093ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LACTb rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LACTb. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LACTb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LACTb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 568384811
• NCBI GeneID: 114294
• NCBI Accession #: NP_001275514.1
• NCBI GenBank Nucleotide #: NM_001288585.1
• UniProt Accession #: P83111; P83096
• Molecular Weight: 41,239 Da
• NCBI Official Full Name: serine beta-lactamase-like protein LACTB, mitochondrial isoform c
• NCBI Official Synonym Full Names: lactamase beta
• NCBI Official Symbol: LACTB
• NCBI Official Synonym Symbols: G24; MRPL56
• NCBI Protein Information: serine beta-lactamase-like protein LACTB, mitochondrial
• UniProt Protein Name: Serine beta-lactamase-like protein LACTB, mitochondrial
• Protein Family: Serine beta-lactamase-like protein
• UniProt Gene Name: LACTB

NCBI Description

This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Dec 2013]

Uniprot Description

Mitochondrial serine protease that acts as a regulator of mitochondrial lipid metabolism (PubMed:28329758). Acts by decreasing protein levels of PISD, a mitochondrial enzyme that converts phosphatidylserine (PtdSer) to phosphatidylethanolamine (PtdEtn), thereby affecting mitochondrial lipid metabolism (PubMed:28329758). It is unclear whether it acts directly by mediating proteolysis of PISD or by mediating proteolysis of another lipid metabolism protein (PubMed:28329758). Acts as a tumor suppressor that has the ability to inhibit proliferation of multiple types of breast cancer cells: probably by promoting decreased levels of PISD, thereby affecting mitochondrial lipid metabolism (PubMed:28329758).