Rabbit ITGB1 (Phospho-Tyr795) Polyclonal Antibody | anti-ITGB1 antibody

ITGB1 (Phospho-Tyr795) Antibody

Cat. Num. AAA9214344

• Gene Names: ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ITGB1 (Phospho-Tyr795); Polyclonal Antibody; ITGB1 (Phospho-Tyr795) Antibody; Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD29; ITGB1; FNRB; MDF2; MSK12; anti-ITGB1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 801

• Applicable Applications for anti-ITGB1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: synthetic peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells treated with H2O2, using ITGB1 (Phospho-Tyr795) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from cos-7 cells (Lane 2), using ITGB1 (Phospho-Tyr795) Antibody. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-ITGB1 antibody

Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion.

Product Categories/Family for anti-ITGB1 antibody

Stem Cells; Phospho Antibodies

References

Argraves W.S.,et al.J. Cell Biol. 105:1183-1190(1987).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Deloukas P.,et al.Nature 429:375-381(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 19743813
• NCBI GeneID: 3688
• NCBI Accession #: NP_002202.2
• NCBI GenBank Nucleotide #: NM_002211.3
• UniProt Accession #: P05556; P78466; P78467; Q13089; Q13090; Q13091; Q13212; A8K6N2; D3DRX9; D3DRY3; D3DRY4; D3DRY5
• Molecular Weight: 88415
• NCBI Official Full Name: integrin beta-1 isoform 1A
• NCBI Official Synonym Full Names: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• NCBI Official Symbol: ITGB1
• NCBI Official Synonym Symbols: CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• NCBI Protein Information: integrin beta-1
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: ITGB1
• UniProt Synonym Gene Names: FNRB; MDF2; MSK12; GPIIA
• UniProt Entry Name: ITB1_HUMAN

NCBI Description

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: cell surface; focal adhesion; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; hemidesmosome; membrane; lamellipodium; cytoplasm; melanosome; plasma membrane; basement membrane; neuromuscular junction; integrin complex; sarcolemma; receptor complex; filopodium; cleavage furrow; external side of plasma membrane

Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; alpha-actinin binding; actin binding; peptide binding; protein kinase binding; integrin binding; viral receptor activity; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding

Biological Process: extracellular matrix organization and biogenesis; maternal process involved in pregnancy; regulation of cell cycle; axon extension; positive regulation of apoptosis; mesodermal cell differentiation; positive regulation of endocytosis; cardiac muscle cell differentiation; leukocyte adhesion; transforming growth factor beta receptor signaling pathway; germ cell migration; tissue homeostasis; response to drug; dendrite morphogenesis; protein transport within lipid bilayer; negative regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; B cell differentiation; cell-cell adhesion mediated by integrin; response to activity; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; axon guidance; entry of virus into host cell; cell-matrix adhesion; cell fate specification; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; visual learning; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; cell migration; regulation of immune response; in utero embryonic development; sarcomere organization; cell-substrate adhesion; cellular calcium ion homeostasis; formation of radial glial scaffolds; heterotypic cell-cell adhesion; negative regulation of Rho protein signal transduction; calcium-independent cell-matrix adhesion; stress fiber formation; cellular defense response; leukocyte tethering or rolling; blood coagulation; homophilic cell adhesion; positive regulation of cell migration