Testing Data
UIM-UBA protein
UIM-UBA Fusion Protein
Purity
>=95% by SDS-PAGE analysis.
Synonyms
UIM-UBA; UIM-UBA Fusion Protein; UIM-UBA protein
Host
E Coli
Purity/Purification
>=95% by SDS-PAGE analysis.
Form/Format
Formulation: 250 ul of 0.4 mg/ml in PBS containing 0.1% azide.
Application Notes
Applications: ; As negative control for biotin UIM-UBA in detecting polyubiquitinated proteins in cell lysate/tissue homogenate using Dot blot.
Preparation and Storage
Store at 4 degree C for 12 months.
Testing Data
Testing Data
Related Product Information for UIM-UBA protein
Ubiquitin is a highly conserved 76-amino acid protein. It can be conjugated via its C-terminus to the amine groups of lysine residues on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin; utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. Different types of polyubiquitin chains can form; depending on the internal lysine residue used for this conjugation. These polyubiquitin chains further can attach to proteins post-translationally and aid in numerous downstream activities like proteasome-mediated proteolysis; autophagy; DNA damage tolerance; inflammation; apoptosis; signal transduction etc. Several classes of Ubiquitin interacting proteins help in mediating these downstream effects. Ubiquitin Interacting Motifs (UIM) and Ubiquitin Associated Domains (UBA) are two large classes of such protein domains which strongly interact with polyubiquitin chains. We have developed an UIM-UBA chimeric protein from the UIM and UBA domains. Both these proteins are well-characterized for their high-affinity interaction with different types of polyubiquitin chains; but not with monomeric ubiquitin.
Product Categories/Family for UIM-UBA protein
Similar Products
Product Notes
The UIM-UBA (Catalog #AAA845511) is a Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Applications: ; As negative control for biotin UIM-UBA in detecting polyubiquitinated proteins in cell lysate/tissue homogenate using Dot blot. Researchers should empirically determine the suitability of the UIM-UBA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UIM-UBA, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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