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Western Blot (WB) (Western BlotPositive WB detected in: K562 whole cell lysate,Mouse brain tissueAll lanes: SLU7 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 69 KDaObserved band size: 69 KDa)

Rabbit anti-Human, Mouse SLU7 Polyclonal Antibody | anti-SLU7 antibody

SLU7 Antibody

Gene Names
SLU7; 9G8; hSlu7
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
SLU7; Polyclonal Antibody; SLU7 Antibody; Pre-mRNA-splicing factor SLU7; hSlu7; anti-SLU7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
586
Applicable Applications for anti-SLU7 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant Human Pre-mRNA-splicing factor SLU7 protein (138-310AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10828 / sc-10829 / sc-10831 / sc-292633 / sc-365660
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: K562 whole cell lysate,Mouse brain tissueAll lanes: SLU7 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 69 KDaObserved band size: 69 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: K562 whole cell lysate,Mouse brain tissueAll lanes: SLU7 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 69 KDaObserved band size: 69 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7049176 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using MBS7049176 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7049176 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7049176 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of A549 cells using MBS7049176 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of A549 cells using MBS7049176 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-SLU7 antibody
Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,387 Da
NCBI Official Full Name
pre-mRNA-splicing factor SLU7
NCBI Official Synonym Full Names
SLU7 homolog, splicing factor
NCBI Official Symbol
SLU7
NCBI Official Synonym Symbols
9G8; hSlu7
NCBI Protein Information
pre-mRNA-splicing factor SLU7
UniProt Protein Name
Pre-mRNA-splicing factor SLU7
Protein Family
UniProt Gene Name
SLU7
UniProt Synonym Gene Names
hSlu7
UniProt Entry Name
SLU7_HUMAN

NCBI Description

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq, Jul 2008]

Uniprot Description

SLU7: Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'- splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation. Belongs to the SLU7 family.

Protein type: RNA processing; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: cytoplasm; intracellular membrane-bound organelle; membrane; nuclear speck; nucleoplasm; nucleus; small nuclear ribonucleoprotein complex; spliceosome

Molecular Function: pre-mRNA 3'-splice site binding; protein binding; second spliceosomal transesterification activity; zinc ion binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome; intracellular protein transport; mRNA 3'-end processing; mRNA export from nucleus; nuclear mRNA 3'-splice site recognition; nuclear mRNA splicing, via spliceosome; RNA export from nucleus; RNA splicing, via transesterification reactions; termination of RNA polymerase II transcription

Research Articles on SLU7

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Product Notes

The SLU7 slu7 (Catalog #AAA7049176) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLU7 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLU7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 500-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the SLU7 slu7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLU7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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