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Immunocytochemistry/Immunofluorescence (ICC/IF))
Mouse RPA70 Monoclonal Antibody | anti-RPA70 antibody
Anti-RPA70 Antibody (8C3-D12-H10)
Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Affinity purification
Synonyms
RPA70; Monoclonal Antibody; Anti-RPA70 Antibody (8C3-D12-H10); Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein; RPA1; anti-RPA70 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
8C3-D12-H10
Specificity
Monoclonal antibody to detect endogenous levels of RPA70. Does not cross-react with related proteins.
Purity/Purification
Affinity purification
Form/Format
Colorless liquid. In PBS (pH7.4) containing with 0.02% sodium azide and 50%
Applicable Applications for anti-RPA70 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
WB: 1:1000
IF: 1:100
IF: 1:100
Immunogen
Recombinant human RPA70 protein fragments expressed in E Coli
Positive Control
WB: Hela, 293T, C6, 3T3 and K562 cell lysates; WB: Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates; IP: HeLa cell lysates; IF: HeLa cells
Preparation and Storage
Store at -20 degree C
The antibody solution should be gently mixed before use
Shelf life: ~12 months
The antibody solution should be gently mixed before use
Shelf life: ~12 months
Immunocytochemistry/Immunofluorescence (ICC/IF))
Immunocytochemistry/Immunofluorescence (ICC/IF))
Testing Data
Testing Data
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-RPA70 antibody
Replication protein A 70 kDa DNA-binding subunit plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage.
Product Categories/Family for anti-RPA70 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
616
NCBI Official Full Name
replication protein A 70 kDa DNA-binding subunit
NCBI Official Synonym Full Names
replication protein A1, 70kDa
NCBI Official Symbol
RPA1
NCBI Official Synonym Symbols
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
NCBI Protein Information
replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; replication factor A protein 1; single-stranded DNA-binding protein
UniProt Protein Name
Replication protein A 70 kDa DNA-binding subunit
UniProt Gene Name
RPA1
UniProt Synonym Gene Names
REPA1; RPA70; RP-A p70
UniProt Entry Name
RFA1_HUMAN
Similar Products
Product Notes
The RPA70 rpa1 (Catalog #AAA847190) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPA70 Antibody (8C3-D12-H10) reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's RPA70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). WB: 1:1000 IF: 1:100. Researchers should empirically determine the suitability of the RPA70 rpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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