Mouse Ret Monoclonal Antibody | anti-Ret antibody

Ret antibody

Cat. Num. AAA831231

• Gene Names: RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• Applications: ELISA, Western Blot
• Synonyms: Ret; Monoclonal Antibody; Ret antibody; Monoclonal Ret; Anti-Ret; c-Ret; Anti Ret; Ret proto-oncogene; anti-Ret antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6E4C4
• Form/Format: Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 90

• Applicable Applications for anti-Ret antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:10,000; WB: 1:500-1:2000
• Biological Significance: Ret is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound ligand-binding GDNF family receptors. Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung?s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity.
• Cross-Reactivity: Human
• Immunogen: Ret antibody was raised in Mouse using a purified recombinant fragment of Ret (aa896-1063) expressed in E Coli as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot

(Western Blot analysis using Ret antibodyWestern Blot showing RET antibody used against truncated RET recombinant protein (1) and RET (aa658-1063)-hIgGFc transfected CHO-K1 cell lysate (2).)

Related Product Information for anti-Ret antibody

Mouse monoclonal Ret antibody

Product Categories/Family for anti-Ret antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 1946207
• NCBI GeneID: 5979
• UniProt Accession #: Q9UQV8; P07949
• Molecular Weight: 10,172 Da
• NCBI Official Full Name: ret, partial
• NCBI Official Synonym Full Names: ret proto-oncogene
• NCBI Official Symbol: RET
• NCBI Official Synonym Symbols: PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase receptor Ret
• UniProt Protein Name: Ret protein
• Protein Family: Retbindin
• UniProt Gene Name: ret
• UniProt Entry Name: Q9UQV8_HUMAN

NCBI Description

This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]

Uniprot Description

Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Oncoprotein; Membrane protein, integral; EC 2.7.10.1; TK group; Ret family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; endosome membrane; receptor complex; lipid raft

Molecular Function: protein binding; protein-tyrosine kinase activity; calcium ion binding; receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; positive regulation of cell size; positive regulation of transcription, DNA-dependent; membrane protein proteolysis; response to pain; regulation of axonogenesis; signal transduction; protein amino acid phosphorylation; enteric nervous system development; neuron maturation; ureteric bud development; embryonic epithelial tube formation; neuron adhesion; neural crest cell migration; response to drug; caspase activation; regulation of cell adhesion; posterior midgut development; positive regulation of neuron maturation; MAPKKK cascade; positive regulation of cell adhesion mediated by integrin; retina development in camera-type eye; homophilic cell adhesion; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

Disease: Multiple Endocrine Neoplasia, Type Iib; Multiple Endocrine Neoplasia, Type Iia; Thyroid Carcinoma, Papillary; Hirschsprung Disease, Susceptibility To, 1; Thyroid Carcinoma, Familial Medullary