Western Blot (WB)
(Western blot analysis of extracts of Neuro2a cell line, using Ret antibody.)
Rabbit Ret Polyclonal Antibody | anti-RET antibody
Ret Polyclonal Antibody
Gene Names
RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
Ret; Polyclonal Antibody; Ret Polyclonal Antibody; RET; CDHF12; CDHR16; HSCR1; MEN2A ; MEN2B ; MTC1 ; PTC; RETELE1 ; RET51 ;; anti-RET antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
1072
Applicable Applications for anti-RET antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1:500-1: 2000
Immunohistochemistry (IHC): 1:50-1: 200
Immunocytochemistry (ICC): 1:50-1: 100
Immunoprecipitation (IP): 1:20-1: 50
Flow Cytometry (FC/FACS): 1:20-1: 50
Immunohistochemistry (IHC): 1:50-1: 200
Immunocytochemistry (ICC): 1:50-1: 100
Immunoprecipitation (IP): 1:20-1: 50
Flow Cytometry (FC/FACS): 1:20-1: 50
Immunogen
A synthetic -peptide of human Ret
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles
Western Blot (WB)
(Western blot analysis of extracts of Neuro2a cell line, using Ret antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Neuro2a cell line, using Ret antibody.)
Related Product Information for anti-RET antibody
The Ret protooncogene (cRet) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membranebound ligandbinding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell linederived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (24). Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung's disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity (5).
References
1. Airaksinen, M.S. et al. (1999) Mol. Cell. Neurosci. 13, 313325. 2. Takahashi, M. et al. (1989) Oncogene 4, 805806. 3. Manie, S. et al. (2001) Trends Genet. 17, 580589. 4. Tallini, G. and Asa, S. (2001) Adv. Anat. Pathol. 8, 345354. 5. Iwashita, T. et al. (1999) Oncogene 18, 39193922.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
175kDa
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase receptor Ret isoform c
NCBI Official Synonym Full Names
ret proto-oncogene
NCBI Official Symbol
RET
NCBI Official Synonym Symbols
PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
NCBI Protein Information
proto-oncogene tyrosine-protein kinase receptor Ret; proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence; cadherin family member 12; hydroxyaryl-protein kinase; cadherin-related family member 16; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase receptor Ret
Protein Family
UniProt Gene Name
RET
UniProt Synonym Gene Names
CDHF12; CDHR16; PTC; RET51
UniProt Entry Name
RET_HUMAN
Similar Products
Product Notes
The RET ret (Catalog #AAA126079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ret Polyclonal Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ret can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Western Blot (WB): 1:500-1: 2000 Immunohistochemistry (IHC): 1:50-1: 200 Immunocytochemistry (ICC): 1:50-1: 100 Immunoprecipitation (IP): 1:20-1: 50 Flow Cytometry (FC/FACS): 1:20-1: 50. Researchers should empirically determine the suitability of the RET ret for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ret, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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