Human Proto-oncogene tyrosine-protein kinase receptor Ret ELISA Kit | RET elisa kit

Human Proto-oncogene tyrosine-protein kinase receptor Ret ELISA Kit

Cat. Num. AAA761820

• Gene Names: RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• Reactivity: Human
• Synonyms: Proto-oncogene tyrosine-protein kinase receptor Ret; Human Proto-oncogene tyrosine-protein kinase receptor Ret ELISA Kit; RET/Proto-oncogene tyrosine-protein kinase receptor Ret/Cadherin family member 12/Proto-oncogene c-Ret/Hirschsprung disease 1/multiple endocrine neoplasia and medullary thyroid carcinoma 1/ret proto-oncogene/RET transforming sequence/CDHR16/MEN2A/MEN2B/CDHF12/CDHR16/PTC/RET51; RET elisa kit

• Reactivity: Human
• Sequence Length: 1114

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RET elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RET antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RET antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RET amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RET can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10862703
• NCBI GeneID: 5979
• NCBI Accession #: NP_066124.1
• NCBI GenBank Nucleotide #: NM_020975.4
• Molecular Weight: 119,847 Da
• NCBI Official Full Name: proto-oncogene tyrosine-protein kinase receptor Ret isoform a
• NCBI Official Synonym Full Names: ret proto-oncogene
• NCBI Official Symbol: RET
• NCBI Official Synonym Symbols: PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1
• NCBI Protein Information: proto-oncogene tyrosine-protein kinase receptor Ret
• UniProt Protein Name: Proto-oncogene tyrosine-protein kinase receptor Ret
• Protein Family: Proto-oncogene tyrosine-protein kinase receptor
• UniProt Gene Name: RET
• UniProt Synonym Gene Names: CDHF12; CDHR16; PTC; RET51
• UniProt Entry Name: RET_HUMAN

NCBI Description

This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]

Uniprot Description

Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (receptor); Oncoprotein; Membrane protein, integral; Protein kinase, TK; Kinase, protein; EC 2.7.10.1; TK group; Ret family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: axon; cell soma; cytoplasm; dendrite; early endosome; endosome membrane; integral to plasma membrane; intracellular membrane-bound organelle; lipid raft; plasma membrane; receptor complex

Molecular Function: ATP binding; calcium ion binding; protein binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: caspase activation; embryonic epithelial tube formation; enteric nervous system development; homophilic cell adhesion; MAPKKK cascade; membrane protein proteolysis; neural crest cell migration; neuron adhesion; neuron maturation; peptidyl-tyrosine phosphorylation; positive regulation of cell adhesion mediated by integrin; positive regulation of cell migration; positive regulation of cell size; positive regulation of neuron maturation; positive regulation of transcription, DNA-dependent; posterior midgut development; protein amino acid phosphorylation; regulation of axonogenesis; regulation of cell adhesion; response to drug; response to pain; retina development in camera-type eye; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway; ureteric bud development

Disease: Hirschsprung Disease, Susceptibility To, 1; Multiple Endocrine Neoplasia, Type Iia; Multiple Endocrine Neoplasia, Type Iib; Thyroid Carcinoma, Familial Medullary; Thyroid Carcinoma, Papillary