Rabbit RasGRP3 Monoclonal Antibody | anti-RASGRP3 antibody

RasGRP3

Cat. Num. AAA602476

• Gene Names: RASGRP3; GRP3; KIAA0846
• Applications: Western Blot
• Purity: As reported
• Synonyms: RasGRP3; Monoclonal Antibody; Anti -RasGRP3; anti-RASGRP3 antibody

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 8A29
• Specificity: Detects endogenous levels of total RasGRP3 protein.
• Purity/Purification: As reported
• Form/Format: Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 g/ml BSA, 50% glycerol and less than 0.02% sodium azide.

• Applicable Applications for anti-RASGRP3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues near the carboxy teminus of human RasGRP3.
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell types using MBS602476.)

Related Product Information for anti-RASGRP3 antibody

Lymphocyte activation occurs in part through activation of the Ras signaling pathway following lymphocyte receptor stimulation. The RasGRP family of guanine nucleotide exchange factors (GEFs) catalyzes the exchange of GDP for GTP on Ras family small GTPases, promoting their active GTP-bound form. Diacylglycerol (DAG) or phorbol ester binding to RasGRP family members causes their translocation to the cell membrane and stimulates their activity (1,2). While T-Cells express RasGRP1, B-cells express both RasGRP1 and RasGRP3. RasGRP3 is important in linking B-cell receptor (BCR) activation to Ras signaling (3). In response to BCR stimulation, RasGRP3 is phosphorylated at Thr133 by PKC. This phosphorylation event further activates RasGRP3 in response to DAG, which stimulates PKC activity (4,5).

Product Categories/Family for anti-RASGRP3 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 24762239
• NCBI GeneID: 25780
• NCBI Accession #: NP_733772.1
• NCBI GenBank Nucleotide #: NM_170672.2
• UniProt Accession #: Q53SD7; Q8IV61
• Molecular Weight: 78,332 Da
• NCBI Official Full Name: ras guanyl-releasing protein 3 isoform 1
• NCBI Official Synonym Full Names: RAS guanyl releasing protein 3 (calcium and DAG-regulated)
• NCBI Official Symbol: RASGRP3
• NCBI Official Synonym Symbols: GRP3; KIAA0846
• NCBI Protein Information: ras guanyl-releasing protein 3; OTTHUMP00000201243; OTTHUMP00000201245; OTTHUMP00000201303; guanine nucleotide exchange factor for Rap1; calcium and DAG-regulated guanine nucleotide exchange factor III
• UniProt Protein Name: Putative uncharacterized protein RASGRP3
• Protein Family: Ras guanyl-releasing protein
• UniProt Gene Name: RASGRP3
• UniProt Entry Name: Q53SD7_HUMAN

NCBI Description

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Uniprot Description

RASGRP3: Guanine nucleotide exchange factor (GEF) for Ras and Rap1. Belongs to the RASGRP family.

Protein type: GEFs, Ras; GEFs

Chromosomal Location of Human Ortholog: 2p25.1-p24.1

Cellular Component: perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane

Molecular Function: diacylglycerol binding; signal transducer activity; Ras GTPase binding; guanyl-nucleotide exchange factor activity; calcium ion binding; kinase binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: small GTPase mediated signal transduction; Ras protein signal transduction; MAPKKK cascade; positive regulation of GTPase activity