Western Blot (WB)
(Anti-NUMB rabbit polyclonal antibody at 1:500 dilution Lane A: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:71 kDa Observed band size:70 kDa)
Rabbit Numb Polyclonal Antibody | anti-Numb antibody
Anti-Numb Antibody, Rabbit Polyclonal
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
IP: 4-8uL/mg of lysate
Western Blot (WB)
(Anti-NUMB rabbit polyclonal antibody at 1:500 dilution Lane A: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:71 kDa Observed band size:70 kDa)
Western Blot (WB)
(Anti-NUMB rabbit polyclonal antibody at 1:500 dilution Lane A: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:71 kDa Observed band size:70 kDa)
Immunoprecipitation (IP)
(NUMB was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-NUMB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-NUMB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 71 kDa Observed band size: 95 kDa)
Immunoprecipitation (IP)
(NUMB was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-NUMB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-NUMB rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 71 kDa Observed band size: 95 kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
NUMB: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: extrinsic to plasma membrane; focal adhesion; clathrin-coated vesicle; apical part of cell; basolateral plasma membrane; integral to plasma membrane; early endosome; plasma membrane; nucleus
Molecular Function: protein binding; cadherin binding; beta-catenin binding; alpha-catenin binding
Biological Process: positive regulation of polarized epithelial cell differentiation; axon guidance; positive regulation of neurogenesis; Notch signaling pathway; negative regulation of Notch signaling pathway; neuroblast division in the subventricular zone; lateral ventricle development; positive regulation of cell migration
Research Articles on Numb
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Product Notes
The Numb numb (Catalog #AAA8100503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Numb Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Numb can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the Numb numb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Numb, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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