Rabbit anti-Human RYBP Polyclonal Antibody | anti-RYBP antibody

RYBP, NT (RYBP, DEDAF, YEAF1, RING1 and YY1-binding protein, Apoptin-associating protein 1, Death effector domain-associated factor, YY1 and E4TF1-associated factor 1) (Biotin)

Cat. Num. AAA6344570

• Gene Names: RYBP; AAP1; DEDAF; YEAF1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RYBP; Polyclonal Antibody; NT (RYBP; DEDAF; YEAF1; RING1 and YY1-binding protein; Apoptin-associating protein 1; Death effector domain-associated factor; YY1 and E4TF1-associated factor 1) (Biotin); YY1 and E4TF1-associated factor 1; anti-RYBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RYBP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RYBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RYBP.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RYBP Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RYBP antibody detected the RYBP protein (arrow).)

Western Blot (WB)

(Western blot analysis of RYBP (arrow) using rabbit polyclonal RYBP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RYBP gene.)

Related Product Information for anti-RYBP antibody

RYBP may be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. In tumor cell lines, may induce apoptosis.

Product Categories/Family for anti-RYBP antibody

Antibodies; Apoptosis

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Chen, D., et al. EMBO Rep. 10(2):166-172(2009). Novak, R.L., et al. Cancer Gene Ther. 15(11):713-722(2008). Danen-van Oorschot, A.A., et al. Cell Death Differ. 11(5):564-573(2004). Guelen, L., et al. Oncogene 23(5):1153-1165(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 112363092
• NCBI GeneID: 23429
• NCBI Accession #: NP_036366.3
• NCBI GenBank Nucleotide #: NM_012234.6
• UniProt Accession #: Q8N488; Q9P2W5; Q9UMW4
• Molecular Weight: 24,822 Da
• NCBI Official Full Name: RING1 and YY1-binding protein
• NCBI Official Synonym Full Names: RING1 and YY1 binding protein
• NCBI Official Symbol: RYBP
• NCBI Official Synonym Symbols: AAP1; DEDAF; YEAF1
• NCBI Protein Information: RING1 and YY1-binding protein; APAP-1; DED-associated factor; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1; apoptin-associating protein 1; death effector domain-associated factor; ring1 interactor RYBP
• UniProt Protein Name: RING1 and YY1-binding protein
• Protein Family: RING1 and YY1-binding protein
• UniProt Gene Name: RYBP
• UniProt Synonym Gene Names: DEDAF; YEAF1; APAP-1; DED-associated factor
• UniProt Entry Name: RYBP_HUMAN

Uniprot Description

RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: nucleoplasm; cytoplasm; PcG protein complex

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; apoptosis; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter