Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human Rac 1 protein Lane 2.: Recombinant human Rac 2 protein Lane 3.: Recombinant human Rac 3 protein)
Mouse anti-Human Rac1,2,3 Monoclonal Antibody | anti-Rac1 antibody
Rac1,2,3
Gene Names
RAC2; Gx; EN-7; HSPC022; p21-Rac2
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
Rac1; 2; 3; Monoclonal Antibody; Ras-related C3 botulinum toxin substrate 1 isoform Rac1; RAC1; p21-Rac1; Ras-like protein TC25; Cell migration-inducing gene 5 protein; Ras-related C3 botulinum toxin substrate 1; Ras-related C3 botulinum toxin substrate 1 isoform Rac1 MGC111543; MIG 5; MIG5; Migration inducing gene 5; Migration inducing protein 5; p21 Rac1; Rac 1; Ras like protein TC25; Ras related C3 botulinum toxin substrate 1; Rho family small GTP binding protein Rac1; TC 25; Ras-related C3 botulinum toxin substrate 2.; EN-7; Gx; HSPC022; Ras-related C3 botulinum toxin substrate 2; EN 7; EN7; HSPC 022; p21 Rac 2; p21 Rac2; p21Rac2; RAC 2; Ras related C3 botulinum toxin substrate 2; Ras related C3 botulinum toxin substrate 3; Rho family small GTP binding protein Rac 2; Rho family small GTP binding protein Rac2; Small G protein; as-related C3 botulinum toxin substrate 3; p21-Rac3; Rac1B; RAS related C3 botulinum substrate 3; rho family small GTP binding protein Rac3; Rho family small GTP binding protein Rac3; RP23-84C12.18; anti-Rac1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT2G10
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
192
Applicable Applications for anti-Rac1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity.
Antigen Species
Human
Immunogen
Recombinant human RAC2 (1-189aa) protein purified from E. coli
Conjugation
Unconjugated
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human Rac 1 protein Lane 2.: Recombinant human Rac 2 protein Lane 3.: Recombinant human Rac 3 protein)
Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human Rac 1 protein Lane 2.: Recombinant human Rac 2 protein Lane 3.: Recombinant human Rac 3 protein)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of Rac1,2,3 in Jurkat cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of Rac1,2,3 in Jurkat cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Related Product Information for anti-Rac1 antibody
Rac is a subfamily of the Rho family of GTPases, small (~21 kDa) signaling G proteins (more specifically a GTPase). When bound to GTP, Rac is activated. In its activated state, Rac participates in the regulation of cell movement, through its involvement in structural changes to the actin Cytoskeleton. Rac is small GTPases that have been linked to multiple human cancers and are implicated in epithelial to mesenchymal transition, cell-cycle progression,migration/invasion, tumor growth, angiogenesis, and oncogenic transformation.
Product Categories/Family for anti-Rac1 antibody
References
Ridley AJ. (2006). Trends Cell Biol. 16(10):522-529.; Maldonado MDM., et al. (2018). Cancer Res. 15; 78(12):3101-3111.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ras-related C3 botulinum toxin substrate 2
NCBI Official Synonym Full Names
Rac family small GTPase 2
NCBI Official Symbol
RAC2
NCBI Official Synonym Symbols
Gx; EN-7; HSPC022; p21-Rac2
NCBI Protein Information
ras-related C3 botulinum toxin substrate 2
UniProt Protein Name
Ras-related C3 botulinum toxin substrate 2
Protein Family
UniProt Gene Name
RAC2
UniProt Entry Name
RAC2_HUMAN
NCBI Description
This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. [provided by RefSeq, Jul 2013]
Research Articles on Rac1
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Product Notes
The Rac1 rac2 (Catalog #AAA201231) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rac1,2,3 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rac1,2,3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Researchers should empirically determine the suitability of the Rac1 rac2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rac1,2,3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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