Human PPP2R4 (Serine/threonine-protein phosphatase 2A activator) ELISA Kit | PPP2R4 elisa kit

Human PPP2R4 (Serine/threonine-protein phosphatase 2A activator) ELISA Kit

Cat. Num. AAA7607220

• Gene Names: Ptpa; PR53; C77440; N28142; Ppp2r4; 2610042B21Rik
• Reactivity: Human
• Synonyms: PPP2R4 (Serine/threonine-protein phosphatase 2A activator); Human PPP2R4 (Serine/threonine-protein phosphatase 2A activator) ELISA Kit; PPP2R4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of. No significant cross-reactivity or interference between and analogues was observed.
• Sequence Length: 251

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPP2R4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1383485766
• NCBI GeneID: 110854
• NCBI Accession #: NP_001349663.1
• NCBI GenBank Nucleotide #: NM_001362734.1
• UniProt Accession #: P58389
• Molecular Weight: 36,710 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A activator isoform 3
• NCBI Official Synonym Full Names: protein phosphatase 2 protein activator
• NCBI Official Symbol: Ptpa
• NCBI Official Synonym Symbols: PR53; C77440; N28142; Ppp2r4; 2610042B21Rik
• NCBI Protein Information: serine/threonine-protein phosphatase 2A activator
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A activator
• UniProt Gene Name: Ptpa
• UniProt Synonym Gene Names: Ppp2r4; PTPA

Uniprot Description

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg2+. Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg2+ (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A ().