Rat Calbindin (CALB) ELISA Kit | CALB elisa kit

Rat Calbindin (CALB) ELISA Kit

Cat. Num. AAA2706113

• Gene Names: CALB1; CALB; D-28K
• Reactivity: Rat
• Synonyms: Calbindin (CALB); Rat Calbindin (CALB) ELISA Kit; CAB27; Calbindin 1; 28kDa; Calbindin D28; Vitamin D-dependent calcium-binding protein; avian-type; CALB elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calbindin (CALB).; No significant cross-reactivity or interference between Calbindin (CALB) and analogues was observed.
• Sequence Length: 204

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Urine and Other Biological Fluids
• Detection Range: 9.38-600pg/mL
• Sensitivity: < 3.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calbindin (CALB) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calbindin (CALB) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CALB elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calbindin (CALB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calbindin (CALB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calbindin (CALB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calbindin (CALB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4826655
• NCBI GeneID: 793
• NCBI Accession #: NP_004920.1
• NCBI GenBank Nucleotide #: NM_004929.3
• UniProt Accession #: P05937; B2R696; B7Z9J4
• Molecular Weight: 23,609 Da
• NCBI Official Full Name: calbindin
• NCBI Official Synonym Full Names: calbindin 1
• NCBI Official Symbol: CALB1
• NCBI Official Synonym Symbols: CALB; D-28K
• NCBI Protein Information: calbindin
• UniProt Protein Name: Calbindin
• Protein Family: Coniferyl aldehyde dehydrogenase
• UniProt Gene Name: CALB1
• UniProt Synonym Gene Names: CAB27

NCBI Description

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]

Uniprot Description

Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.