Testing Data
(C3H/He mouse splenocytes were stained with purified H-2Dk (filled histogram) or mouse IgG2a,k isotype control (open histogram), followed by anti-mouse IgG2a FITC.)
Mouse anti-Mouse MHC Class 1, H-2Dk Monoclonal Antibody | anti-H2-K1 antibody
MHC Class 1, H-2Dk (MHC Class I)
Gene Names
H2-K1; K-f; H-2K; H2-K; H-2K(d)
Reactivity
Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
MHC Class 1; H-2Dk; Monoclonal Antibody; H-2Dk (MHC Class I); Anti -MHC Class 1; anti-H2-K1 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Specificity
Recognizes H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. Weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-H2-K1 antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry and Immunoprecipitation.
Dilution: Flow Cytometry: 1ug/10e6 cells in 100ul volume
Dilution: Flow Cytometry: 1ug/10e6 cells in 100ul volume
Immunogen
C3H mouse splenocytes
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(C3H/He mouse splenocytes were stained with purified H-2Dk (filled histogram) or mouse IgG2a,k isotype control (open histogram), followed by anti-mouse IgG2a FITC.)
Testing Data
(C3H/He mouse splenocytes were stained with purified H-2Dk (filled histogram) or mouse IgG2a,k isotype control (open histogram), followed by anti-mouse IgG2a FITC.)
Related Product Information for anti-H2-K1 antibody
This antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.
Product Categories/Family for anti-H2-K1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,302 Da
NCBI Official Full Name
MHC H-2K-k protein
NCBI Official Synonym Full Names
histocompatibility 2, K1, K region
NCBI Official Symbol
H2-K1
NCBI Official Synonym Symbols
K-f; H-2K; H2-K; H-2K(d)
NCBI Protein Information
H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC class I antigen; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen; class I major histocompatibility antigen H-2Kd; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain
UniProt Protein Name
H-2 class I histocompatibility antigen, K-B alpha chain
Protein Family
UniProt Gene Name
H2-K1
UniProt Synonym Gene Names
H2-K; H-2K(B)
UniProt Entry Name
HA1B_MOUSE
Uniprot Description
HLAC iso22: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.
Protein type: Receptor, misc.; Membrane protein, integral
Cellular Component: Golgi apparatus; cell surface; membrane; endoplasmic reticulum; integral to membrane; MHC class I protein complex; Golgi medial cisterna; external side of plasma membrane
Molecular Function: protein binding; peptide antigen binding; protein heterodimerization activity; beta-2-microglobulin binding; T cell receptor binding; peptide binding; TAP binding; receptor binding
Biological Process: antigen processing and presentation; antigen processing and presentation of peptide antigen via MHC class I; immune system process; positive regulation of T cell mediated cytotoxicity; defense response to bacterium; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; inner ear development; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
Research Articles on H2-K1
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Product Notes
The H2-K1 h2-k1 (Catalog #AAA6009992) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MHC Class 1, H-2Dk (MHC Class I) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MHC Class 1, H-2Dk can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry and Immunoprecipitation. Dilution: Flow Cytometry: 1ug/10e6 cells in 100ul volume. Researchers should empirically determine the suitability of the H2-K1 h2-k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC Class 1, H-2Dk, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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