Western Blot (WB)
(Western blot detection of Phospho-PRC1 (Thr481) in Hela,Jurkat cell lysates using Phospho-PRC1 (Thr481) Rabbit mAb(1:500 diluted). Predicted band size:72kDa. Observed band size:72kDa.)
Rabbit anti-Human PRC1 Monoclonal Antibody | anti-PRC1 antibody
Phospho-PRC1 (Thr481) Rabbit mAb
IHC: 1:50
ICC/IF: 1:50
Western Blot (WB)
(Western blot detection of Phospho-PRC1 (Thr481) in Hela,Jurkat cell lysates using Phospho-PRC1 (Thr481) Rabbit mAb(1:500 diluted). Predicted band size:72kDa. Observed band size:72kDa.)
Western Blot (WB)
(Western blot detection of Phospho-PRC1 (Thr481) in Hela,Jurkat cell lysates using Phospho-PRC1 (Thr481) Rabbit mAb(1:500 diluted). Predicted band size:72kDa. Observed band size:72kDa.)
Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
Uniprot Description
PRC1: translocates to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. Acts as a microtubule-binding and bundling protein both in vivo and vitro. Interacts with the C-terminal Rho-GAP domain and the basic region of MgcRacGAP. The interaction with MgcRacGAP inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. Interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis.
Protein type: Cell cycle regulation; Cytoskeletal; Microtubule-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 15q26.1
Cellular Component: nucleoplasm; microtubule cytoskeleton; spindle pole; spindle microtubule; cytoplasm; plasma membrane; spindle; nucleus
Molecular Function: identical protein binding; protein binding; kinesin binding; microtubule binding; protein kinase binding
Biological Process: mitotic spindle elongation; cytokinesis
Research Articles on PRC1
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Product Notes
The PRC1 prc1 (Catalog #AAA4752972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PRC1 (Thr481) Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 IHC: 1:50 ICC/IF: 1:50. Researchers should empirically determine the suitability of the PRC1 prc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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