Rabbit MCT1 Polyclonal Antibody | anti-MCT1 antibody

Rabbit Polyclonal (IgG) to Human MCT1

Cat. Num. AAA241491

• Gene Names: MCTS1; MCT1; MCT-1
• Reactivity: Human, Mouse, Bovine, Dog, Pig, Chicken, Xenopus, Zebrafish
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MCT1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human MCT1; MCTS1; MCT-1; anti-MCT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Bovine, Dog, Pig, Chicken, Xenopus, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Residues 3-14[KKFDEKENVSNC] of the 20 kD MCT-1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 181

• Applicable Applications for anti-MCT1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA; IHC-P (20 ug/ml); WB (1:500 - 1:1000); (Optimal dilution to be determined by the researcher)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human MCTS1 / MCT1. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Elephant, Dog, Bovine, Pig, Opossum, Chicken, Xenopus, Frog, Trout, Catfish, Salmon, Sablefish, Zebrafish (100%); Panda, Smelt (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: MCT1 antibody was raised against synthetic peptide from human MCTS1 / MCT1. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Elephant, Dog, Bovine, Pig, Opossum, Chicken, Xenopus, Frog, Trout, Catfish, Salmon, Sablefish, Zebrafish (100%); Panda, Smelt (92%); Gorilla, Monkey, Marmoset, Rat, Bat, Turkey, Platypus (83%).
• Antigen Modification: aa3-14
• Usage: Immunohistochemistry: MBS241491 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241491 was determined to be 20 ug/ml.
• Preparation and Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-MCT1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 7662502
• NCBI GeneID: 28985
• NCBI Accession #: NP_054779.1
• NCBI GenBank Nucleotide #: NM_014060.2
• UniProt Accession #: Q9ULC4; Q502X6; B4DGY2
• Molecular Weight: 20,550 Da
• NCBI Official Full Name: malignant T-cell-amplified sequence 1 isoform 1
• NCBI Official Synonym Full Names: malignant T cell amplified sequence 1
• NCBI Official Symbol: MCTS1
• NCBI Official Synonym Symbols: MCT1; MCT-1
• NCBI Protein Information: malignant T-cell-amplified sequence 1; multiple copies T-cell malignancies
• UniProt Protein Name: Malignant T-cell-amplified sequence 1
• UniProt Gene Name: MCTS1
• UniProt Synonym Gene Names: MCT1; MCT-1
• UniProt Entry Name: MCTS1_HUMAN

Uniprot Description

MCTS1: Anti-oncogene that play a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma- irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when overexpressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines overexpressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3. Belongs to the MCTS1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: cytoplasm; plasma membrane

Molecular Function: translation initiation factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of growth; positive regulation of cell proliferation; ribosome disassembly; cell cycle; response to DNA damage stimulus; formation of translation preinitiation complex