Western Blot (WB)
(Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: PC-12 Lane 3: HL-60)
Rabbit PDPK1 Monoclonal Antibody | anti-PDPK1 antibody
PDPK1 Antibody
IHC: 1:50-1:200
ICC: 1:100-1:500
Western Blot (WB)
(Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: PC-12 Lane 3: HL-60)
Western Blot (WB)
(Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: PC-12 Lane 3: HL-60)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
Uniprot Description
PDK1: an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described.
Protein type: Nuclear receptor co-regulator; EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; PKB family
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nucleoplasm; cell projection; focal adhesion; membrane; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; phospholipase binding; 3-phosphoinositide-dependent protein kinase activity; kinase activity; protein kinase binding; insulin receptor binding; ATP binding; phospholipase activator activity
Biological Process: focal adhesion formation; regulation of mast cell degranulation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; T cell receptor signaling pathway; protein amino acid phosphorylation; synaptic transmission; regulation of transcription, DNA-dependent; regulation of I-kappaB kinase/NF-kappaB cascade; epidermal growth factor receptor signaling pathway; platelet activation; cell migration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; activation of protein kinase B; calcium-mediated signaling; peptidyl-threonine phosphorylation; hyperosmotic response; cellular response to insulin stimulus; T cell costimulation; insulin receptor signaling pathway; negative regulation of toll-like receptor signaling pathway; negative regulation of protein kinase activity; innate immune response; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of transforming growth factor beta receptor signaling pathway; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway
Research Articles on PDPK1
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Product Notes
The PDPK1 pdpk1 (Catalog #AAA9435908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDPK1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the PDPK1 pdpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDPK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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