Western Blot (WB)
((2ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RDH5 Polyclonal Antibody | anti-RDH5 antibody
Goat anti-RDH5 (aa 19-31) Antibody
Immunohistochemistry: In paraffin embedded Human Retina shows staining of the Ganglion Cell Layer. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 35kDa band observed in Mouse Eye lysates (calculated MW of 34.8kDa according to Mouse NP_598767.1). Recommended concentration: 2-4ug/ml.
Western Blot (WB)
((2ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Retina. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S1 (biogenesis of lysosomal organelles complex-1, subunit 1) gene. [provided by RefSeq, Dec 2010]
Uniprot Description
RDH5: Stereospecific 11-cis retinol dehydrogenase, which catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments. Also able to oxidize 9-cis-retinol and 13-cis-retinol, but not all- trans-retinol. Active in the presence of NAD as cofactor but not in the presence of NADP. Defects in RDH5 are a cause of retinitis punctata albescens (RPA); also known as fundus albipunctatus (FA). RPA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; EC 1.1.1.315; Oxidoreductase
Chromosomal Location of Human Ortholog: 12q13-q14
Cellular Component: endoplasmic reticulum lumen; endoplasmic reticulum membrane
Molecular Function: retinol dehydrogenase activity
Biological Process: response to stimulus; retinoid metabolic process; retinol metabolic process; visual perception
Disease: Fundus Albipunctatus
Research Articles on RDH5
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Product Notes
The RDH5 rdh5 (Catalog #AAA422384) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RDH5 (aa 19-31) Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's RDH5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Retina shows staining of the Ganglion Cell Layer. Recommended concentration, 5-10ug/ml. Western Blot: Approx 35kDa band observed in Mouse Eye lysates (calculated MW of 34.8kDa according to Mouse NP_598767.1). Recommended concentration: 2-4ug/ml. Researchers should empirically determine the suitability of the RDH5 rdh5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RDRQSLPASN AFV. It is sometimes possible for the material contained within the vial of "RDH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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