Rabbit anti-Human Ubiquilin3 Polyclonal Antibody | anti-UBQLN3 antibody

Ubiquilin3, NT (UBQLN3) (MaxLight 550)

Cat. Num. AAA6504569

• Gene Names: UBQLN3; TUP-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Ubiquilin3; Polyclonal Antibody; NT (UBQLN3) (MaxLight 550); anti-UBQLN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Ubiquilin3.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-UBQLN3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human Ubiquilin3, UniProt Accession #Q9H347.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS625443. Ubiquilin3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted with theMBS625443, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS625443 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-UBQLN3 antibody

Antibodies; Disease Markers

References

1. Conklin, D., et al., Gene 249(1-2), 91-98 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 8567418
• NCBI GeneID: 50613
• NCBI Accession #: NP_059509.1
• NCBI GenBank Nucleotide #: NM_017481.2
• UniProt Accession #: Q9H347; Q9NRE0
• Molecular Weight: 70,841 Da
• NCBI Official Full Name: ubiquilin-3
• NCBI Official Synonym Full Names: ubiquilin 3
• NCBI Official Symbol: UBQLN3
• NCBI Official Synonym Symbols: TUP-1
• NCBI Protein Information: ubiquilin-3
• UniProt Protein Name: Ubiquilin-3
• Protein Family: Ubiquilin
• UniProt Gene Name: UBQLN3
• UniProt Entry Name: UBQL3_HUMAN

NCBI Description

Summary: This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This gene is specifically expressed in the testis, and proposed to regulate cell-cycle progression during spermatogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

UBQLN3:

Chromosomal Location of Human Ortholog: 11p15