Mouse anti-Human PAX5 Monoclonal Antibody | anti-PAX5 antibody

Mouse Monoclonal [clone 8F9] (IgG1,k) to Human PAX5

Cat. Num. AAA245998

• Gene Names: PAX5; ALL3; BSAP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: PAX5; Monoclonal Antibody; Mouse Monoclonal [clone 8F9] (IgG1; k) to Human PAX5; Anti-PAX5 Antibody (clone 8F9) IHC-plus; BSAP; Paired box 5; Paired box protein Pax-5; Transcription factor PAX 5; Paired box homeotic gene 5; Human PAX5; anti-PAX5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 8F9
• Specificity: Human PAX5
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 391

• Applicable Applications for anti-PAX5 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: PAX5 (NP_057953, 192 a.a. ~ 302 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: PAX5 antibody was raised against pAX5 (NP_057953, 192 a.a. ~ 302 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-PAX5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(PAX5 monoclonal antibody clone 8F9. Western blot of PAX5 expression in IMR-32.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PAX5 is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 9951920
• NCBI GeneID: 5079
• NCBI Accession #: NP_057953.1
• NCBI GenBank Nucleotide #: NM_016734.2
• UniProt Accession #: Q02548; O75933; Q5SFM2; Q6S728; A3QVP6; A3QVP7; A3QVP8; C0KTF6; C0KTF7; C0KTF8; C0KTF9; C0KTG0
• Molecular Weight: 34,132 Da
• NCBI Official Full Name: paired box protein Pax-5 isoform 1
• NCBI Official Synonym Full Names: paired box 5
• NCBI Official Symbol: PAX5
• NCBI Official Synonym Symbols: ALL3; BSAP
• NCBI Protein Information: paired box protein Pax-5; paired domain gene 5; paired box homeotic gene 5; transcription factor PAX 5; B-cell lineage specific activator
• UniProt Protein Name: Paired box protein Pax-5
• Protein Family: Paired box protein
• UniProt Gene Name: PAX5
• UniProt Synonym Gene Names: BSAP
• UniProt Entry Name: PAX5_HUMAN

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

PAX5: May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. Interacts with DAXX. Binds DNA as a monomer. Binds TLE4.

Protein type: Oncoprotein

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: nucleus

Molecular Function: protein binding

Biological Process: negative regulation of histone H3-K9 methylation; transcription from RNA polymerase II promoter; nervous system development; organ morphogenesis; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; humoral immune response

Disease: Leukemia, Acute Lymphoblastic, Susceptibility To, 3