Goat PAX5/BSAP Polyclonal Antibody | anti-PAX5 antibody

Goat anti-PAX5/BSAP Antibody

Cat. Num. AAA421040

• Gene Names: PAX5; ALL3; BSAP
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PAX5/BSAP; Polyclonal Antibody; Goat anti-PAX5/BSAP Antibody; PAX5; BSAP; paired box gene 5 (B-cell lineage specific activator protein); B-cell lineage specific activator protein; paired box homeotic gene 5 (B-cell lineage specific activator protein); paired box 5; B cell specific activator protein; B-cell lineage s; PAX5 antibody; BSAP antibody; paired box gene 5 (B-cell lineage specific activator protein) antibody; B-cell lineage specific activator protein antibody; paired box homeotic gene 5 (B-cell lineage specific activator protein) antibody; paired box 5 antibody; B cell specific activator protein antibody; B-cell lineage specific activator antibody; paired box homeotic gene 5 antibody; transcription factor PAX 5 antibody; PAX5 / BSAP; anti-PAX5 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DLEKNYPTPRTSR
• Sequence Length: 391

• Applicable Applications for anti-PAX5 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Western Blot: Approx 40kDa band observed in Human Lymph and Human Spleen lysates (calculated MW of 42.1kDa according to NP_057953.1). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence DLEKNYPTPRTSR-C, from the N Terminus of the protein sequence according to NP_057953.1.
• Epitope: N Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Lymph lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 9951920
• NCBI GeneID: 5079
• NCBI Accession #: NP_057953.1
• NCBI GenBank Nucleotide #: NM_016734.2
• Molecular Weight: 34,132 Da
• NCBI Official Full Name: paired box protein Pax-5 isoform 1
• NCBI Official Synonym Full Names: paired box 5
• NCBI Official Symbol: PAX5
• NCBI Official Synonym Symbols: ALL3; BSAP
• NCBI Protein Information: paired box protein Pax-5
• UniProt Protein Name: Paired box protein Pax-5
• Protein Family: Paired box protein
• UniProt Gene Name: PAX5
• UniProt Synonym Gene Names: BSAP
• UniProt Entry Name: PAX5_HUMAN

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

PAX5: May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. Interacts with DAXX. Binds DNA as a monomer. Binds TLE4.

Protein type: Oncoprotein

Chromosomal Location of Human Ortholog: 9p13

Molecular Function: protein binding

Biological Process: humoral immune response; multicellular organismal development; organ morphogenesis; transcription from RNA polymerase II promoter

Disease: Leukemia, Acute Lymphoblastic, Susceptibility To, 3