RNASEH2A recombinant protein

RNASEH2A, 1-299aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA204956

• Gene Names: RNASEH2A; AGS4; JUNB; RNHL; RNHIA; RNASEHI
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: RNASEH2A; 1-299aa; Human; His tag; E Coli (Denatured); Ribonuclease H2 subunit A; AGS4; JuNB; RNASEHI; RNHIA; RNHL; RNASEH2A recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDLSELE RDNTGRCRLS SPVPAVCRKE PCVLGVDEAG RGPVLGPMVY AICYCPLPRL ADLEALKVAD SKTLLESERE RLFAKMEDTD FVGWALDVLS PNLISTSMLG RVKYNLNSLS HDTATGLIQY ALDQGVNVTQ VFVDTVGMPE TYQARLQQSF PGIEVTVKAK ADALYPVVSA ASICAKVARD QAVKKWQFVE KLQDLDTDYG SGYPNDPKTK AWLKEHVEPV FGFPQFVRFS WRTAQTILEK EAEDVIWEDS ASENQEGLRK ITSYFLNEGS QARPRSSHRY FLERGLESAT SL
• Sequence Length: 299

• Applicable Applications for RNASEH2A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RNASEH2A recombinant protein

Ribonuclease H2 subunit A, also known as RNASEH2A, belongs to the RNase HII family and eukaryotic subfamily. Localized to the nucleus, RNASEH2A mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. RNASEH2A catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4). Recombinant human RNASEH2A protein, fused to His-tag at N-terminus, was expressed in E Coli

Product Categories/Family for RNASEH2A recombinant protein

Epigenomics

References

Frank P., et al. (1998) Proc Natl Acad Sci uSA. 95:12872-12877.; Bayliss C D., et al. (2005) Nucleic Acads Res. 33:400-408.

NCBI and Uniprot Product Information

• NCBI GI #: 38455391
• NCBI GeneID: 10535
• NCBI Accession #: NP_006388
• NCBI GenBank Nucleotide #: NM_006397.2
• UniProt Accession #: O75792; Q96F11; B2RCY1
• Molecular Weight: 35.8kDa (322aa)
• NCBI Official Full Name: ribonuclease H2 subunit A
• NCBI Official Synonym Full Names: ribonuclease H2 subunit A
• NCBI Official Symbol: RNASEH2A
• NCBI Official Synonym Symbols: AGS4; JUNB; RNHL; RNHIA; RNASEHI
• NCBI Protein Information: ribonuclease H2 subunit A
• UniProt Protein Name: Ribonuclease H2 subunit A
• Protein Family: Ribonuclease
• UniProt Gene Name: RNASEH2A
• UniProt Synonym Gene Names: RNASEHI; RNHIA; RNase H2 subunit A; AGS4; RNase HI large subunit

NCBI Description

The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]

Uniprot Description

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.