Mouse anti-Human Nucleophosmin Monoclonal Antibody | anti-NPM antibody

Nucleophosmin Monoclonal Antibody

Cat. Num. AAA2563628

• Gene Names: NPM1; B23; NPM
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: Nucleophosmin; Monoclonal Antibody; Nucleophosmin Monoclonal Antibody; B23; MGC104254; NO38; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin); nucleophosmin nucleoplasmin family member 1; Nucleophosmin/nucleoplasmin family member 1; Numatrin; OTTHUMP00000161024; OTTHUMP00000161025; OTTHUMP00000223397; OTTHUMP00000223398; anti-NPM antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: LE0201
• Purity/Purification: Affinity purification
• Form/Format: PBS with 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 259

• Applicable Applications for anti-NPM antibody: Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC)
• Application Notes: IHC: 1:100-1:200
• Immunogen: Full length protein from eukaryotic expression system.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20?. Avoid freeze/ thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue using Nucleophosmin Monoclonal Antibody dilution of 1:200.)

Related Product Information for anti-NPM antibody

NPM1 (Nucleophosmin) is a phosphoprotein which moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. Diseases associated with NPM1 include Leukemia, Acute Myeloid and Lymphomatoid Papulosis. Among its related pathways areValidated targets of C-MYC transcriptional activation and Chromosome Maintenance.

Product Categories/Family for anti-NPM antibody

Cancer, Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 83641870
• NCBI GeneID: 4869
• NCBI Accession #: NP_001032827.1
• NCBI GenBank Nucleotide #: NP_001032827.1
• UniProt Accession #: P06748
• NCBI Official Full Name: nucleophosmin isoform 3
• NCBI Official Synonym Full Names: nucleophosmin 1
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM
• UniProt Entry Name: NPM_HUMAN

NCBI Description

The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Oncoprotein; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal large subunit binding; NF-kappaB binding; protein binding; protein heterodimerization activity; Tat protein binding; ribosomal small subunit binding

Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; DNA repair; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; negative regulation of cell proliferation; nucleosome assembly; regulation of endodeoxyribonuclease activity; protein localization; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis