Rabbit anti-Human, Mouse NPM Polyclonal Antibody | anti-NPM antibody

NPM (Phospho-Thr199) Antibody

Cat. Num. AAA9403222

• Gene Names: NPM1; B23; NPM
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: NPM; Polyclonal Antibody; NPM (Phospho-Thr199) Antibody; B23; anti-NPM antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of NPM only when phosphorylated at Threonine 199.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 259

• Applicable Applications for anti-NPM antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Threonine 199
• Modification: Phospho-Thr199
• Target Name: NPM
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from Mouse heart tissue using NPM (Phospho-Thr199) Antibody.The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-NPM antibody

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.

Product Categories/Family for anti-NPM antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 83641870
• NCBI GeneID: 4869
• NCBI Accession #: NP_001032827.1
• NCBI GenBank Nucleotide #: NM_001037738.2
• UniProt Accession #: P06748; P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6
• Molecular Weight: 28,400 Da
• NCBI Official Full Name: nucleophosmin isoform 3
• NCBI Official Synonym Full Names: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin
• UniProt Protein Name: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM
• UniProt Entry Name: NPM_HUMAN

NCBI Description

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Translation; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal large subunit binding; NF-kappaB binding; protein binding; Tat protein binding; protein heterodimerization activity; ribosomal small subunit binding

Biological Process: regulation of centriole replication; positive regulation of translation; viral reproduction; centrosome cycle; cell aging; nucleocytoplasmic transport; DNA repair; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; negative regulation of cell proliferation; nucleosome assembly; regulation of endodeoxyribonuclease activity; protein localization; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis