Immunofluorescence (IF)
(NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence. Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody.)
Rat anti-Human NCAPH2 Monoclonal Antibody | anti-NCAPH2 antibody
NCAPH2 antibody (mAb)(Clone 5F2G4)
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Protein G Chromatography
Synonyms
NCAPH2; Monoclonal Antibody; NCAPH2 antibody (mAb)(Clone 5F2G4); CAPH2; anti-NCAPH2 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
5F2G4
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-NCAPH2 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
ICC/IF: 1-2ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
ICC/IF: 1-2ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a recombinant protein to human NCAPH2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Immunofluorescence (IF)
(NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence. Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody.)
Immunofluorescence (IF)
(NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence. Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody.)
Western Blot (WB)
(NCAPH2 antibody (mAb) (Clone 5F2G4) tested by Western blot. NCAPH2 antibody detection by Western blot. The analysis was performed using 30 ug of HeLa nuclear extract and NCAPH2 antibody at a 1 ug/ml dilution.)
Western Blot (WB)
(NCAPH2 antibody (mAb) (Clone 5F2G4) tested by Western blot. NCAPH2 antibody detection by Western blot. The analysis was performed using 30 ug of HeLa nuclear extract and NCAPH2 antibody at a 1 ug/ml dilution.)
Related Product Information for anti-NCAPH2 antibody
Short Description: Our NCAPH2 antibody (mAb)(Clone 5F2G4) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples.
Background: NCAPH2 (Non-SMC Condensin II Complex, Subunit H2) Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development. (By similarity)
Background: NCAPH2 (Non-SMC Condensin II Complex, Subunit H2) Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development. (By similarity)
Product Categories/Family for anti-NCAPH2 antibody
NCBI and Uniprot Product Information
Molecular Weight
90kDa
Similar Products
Product Notes
The NCAPH2 (Catalog #AAA389008) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The NCAPH2 antibody (mAb)(Clone 5F2G4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NCAPH2 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ICC/IF: 1-2ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the NCAPH2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCAPH2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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