Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Mouse anti-Human SI (Sucrase-isomaltase, Intestinal, MGC131621, MGC131622) Monoclonal Antibody | anti-SI antibody
SI (Sucrase-isomaltase, Intestinal, MGC131621, MGC131622)
Other applications not tested.
Optimal dilutions to be determined by the researcher.
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Testing Data
(Detection limit for recombinant GST tagged SI is 0.03ng/ml using MBS646764 as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged SI is 0.03ng/ml using MBS646764 as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.[provided by RefSeq, Apr 2010]
Uniprot Description
Function: Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides. Ref.7
Catalytic activity: Hydrolysis of sucrose and maltose by an alpha-D-glucosidase-type action.Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
Subunit structure: The resulting sucrase and isomaltase subunits stay associated with one another in a complex by non-covalent linkages.
Subcellular location: Apical cell membrane; Single-pass type II membrane protein. Note: Brush border.
Tissue specificity: Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. Ref.5
Post-translational modification: The precursor is proteolytically cleaved when exposed to pancreatic proteases in the intestinal lumen.Sulfated
By similarity.
Involvement in disease: Congenital sucrase-isomaltase deficiency (CSID) [MIM:222900]: Autosomal recessive intestinal disorder that is clinically characterized by fermentative diarrhea, abdominal pain, and cramps upon ingestion of sugar. The symptoms are the consequence of absent or drastically reduced enzymatic activities of sucrase and isomaltase. The prevalence of CSID is 0.02 % in individuals of European descent and appears to be much higher in Greenland, Alaskan, and Canadian native people. CSID arises due to post-translational perturbations in the intracellular transport, polarized sorting, aberrant processing, and defective function of SI.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Miscellaneous: There is a high degree of homology between the isomaltase and sucrase portions (41% of amino acid identity) indicating that this protein is evolved by partial gene duplication.
Sequence similarities: Belongs to the glycosyl hydrolase 31 family.Contains 2 P-type (trefoil) domains.
Research Articles on SI
Similar Products
Product Notes
The SI si (Catalog #AAA646764) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SI (Sucrase-isomaltase, Intestinal, MGC131621, MGC131622) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SI (Sucrase-isomaltase, Intestinal, MGC131621, MGC131622) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the SI si for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DGESIDTYER DLYLSVQFNL NQTTLTSTIL KRGYINKSET RLGSLHVWGK GTTPVNAVTL TYNGNKNSLP FNEDTTNMIL RIDLTTHNVT LEEPIEINW*. It is sometimes possible for the material contained within the vial of "SI (Sucrase-isomaltase, Intestinal, MGC131621, MGC131622), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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