Flow Cytometry (FC/FACS)
(Staining of C57BL/6 bone marrow cells with CD45R antibody (FITC) and 0.125 ug of Armenian Hamster IgG Isotype Control (biotin) (left) or 0.06 ug of CD29 antibody (biotin) (right) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Hamster CD29 Monoclonal Antibody | anti-CD29 antibody
CD29 antibody (biotin)
Gene Names
ITGB1; CD29
Applications
Flow Cytometry, Functional Assay
Synonyms
CD29; Monoclonal Antibody; CD29 antibody (biotin); Monoclonal CD29; Anti-CD29; ITGB1; Integrin beta 1; CD 29; platelet GPIIa; CD-29; anti-CD29 antibody
Host
Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
HMb1-1
Specificity
Mouse, Rat
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
135
Applicable Applications for anti-CD29 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.25 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The HMb1-1 monoclonal antibody reacts with mouse and rat CD29, a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These alphabeta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 bone marrow cells with CD45R antibody (FITC) and 0.125 ug of Armenian Hamster IgG Isotype Control (biotin) (left) or 0.06 ug of CD29 antibody (biotin) (right) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 bone marrow cells with CD45R antibody (FITC) and 0.125 ug of Armenian Hamster IgG Isotype Control (biotin) (left) or 0.06 ug of CD29 antibody (biotin) (right) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Related Product Information for anti-CD29 antibody
Armenian Hamster monoclonal CD29 antibody (biotin)
Product Categories/Family for anti-CD29 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,701 Da
NCBI Official Full Name
CD29, partial
NCBI Official Symbol
ITGB1
NCBI Official Synonym Symbols
CD29
NCBI Protein Information
integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
UniProt Protein Name
CD29
UniProt Entry Name
A8CUR0_HORSE
Similar Products
Product Notes
The CD29 (Catalog #AAA833607) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD29 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.25 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD29 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD29, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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