Mouse anti-Human NF-kB p65 Monoclonal Antibody | anti-RELA antibody

NF-kB p65 (Nuclear Factor kB)

Cat. Num. AAA602308

• Reactivity: Human
• Applications: ELISA, Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NF-kB p65; Monoclonal Antibody; NF-kB p65 (Nuclear Factor kB); Anti -NF-kB p65 (Nuclear Factor kB); anti-RELA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3G209
• Specificity: Specific for NF-kB (p65). Crossreactivity with related proteins has not been observed. Species Sequence Homology: Based on amino acid homology, reactivity with mouse and rat NFkB is likely.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-RELA antibody: ELISA (EL/EIA), Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in ELISA, Western Blot and Gel Mobility Shift Assay.; Dilution: ELISA: 0.1-1.0ug/ml; Western Blot: 1ug/ml; Gel Mobility Shift Assay: 5-10ug/ml
• Immunogen: Recombinant protein (~175 amino acids) from the C-terminal portion of human NF-kB (p65).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-RELA antibody

The NF-kB family of induced-activated transcription factors are dimers comprised of members of the NF-kB /rel protein family. Activation of NF-kB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA, LPS, UV light, and others. In most cell types, NF-kB is present in the cytoplasm as a 50kD (p50) subunit and a 65kD (p65, rel A) subunit. In unstimulated cells, nuclear localization signals present on p65 are masked by members of the Ik-B family of inhibitory proteins. Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosome-dependent pathway, allowing nuclear translocation of the active dimeric NF-kB transcription factor. In the nucleus, NF-kB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.

Product Categories/Family for anti-RELA antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 460974
• NCBI GeneID: 396027
• UniProt Accession #: P98152
• Molecular Weight: 60,072 Da
• NCBI Official Full Name: NF-kB p65 subunit
• NCBI Official Symbol: RELA
• NCBI Protein Information: transcription factor p65; NF-kB p65 subunit; nuclear factor NF-kappa-B p65 subunit
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: RELA
• UniProt Entry Name: TF65_CHICK

Uniprot Description

Function: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1

Subunit structure: Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-c-Rel complex. Component of the NF-kappa-B p65-p105 complex.

Subcellular location: Nucleus. Cytoplasm. Note: Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).

Tissue specificity: Spleen; lower level in brain.

Sequence similarities: Contains 1 RHD (Rel-like) domain.