Mouse anti-Human, Mouse NFkB, p65 Monoclonal Antibody | anti-NKIRAS2 antibody

NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)

Cat. Num. AAA633086

• Gene Names: NKIRAS2; KBRAS2; kappaB-Ras2
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: NFkB; p65; Monoclonal Antibody; p65 (NF-kB; Nuclear Factor kappa B; RelA); Anti -NFkB; anti-NKIRAS2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 4H211
• Specificity: Recognizes human NFkB p65. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

• Applicable Applications for anti-NKIRAS2 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot and Flow Cytometry.; Dilution: Western Blot: 2ug/ml, A band of ~65kD should be detected.; Flow Cytometry: 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.
• Positive Control: Ramos, Daudi, HeLa, NIH 3T3
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of p65 using MBS633086 (2ug/ml) in 30ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.)

Immunohistochemistry (IHC)

(Immunohistochemistry in Paraffin Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed MBS633086 (5ug/ml). Human tissue TMA was used for this test.S taining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.)

Flow Cytometry (FC/FACS)

(Flow Cytometry: Intracellular staining of 293 HEK cells using MBS633086 (0.5ug). Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.)

Testing Data

(Simple Western lane view shows a specific band for NFkB p65 using MBS633086 in 1mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kD separation system.)

Related Product Information for anti-NKIRAS2 antibody

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Product Categories/Family for anti-NKIRAS2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 222136632
• NCBI GeneID: 28511
• NCBI Accession #: NP_001138401.1
• NCBI GenBank Nucleotide #: NM_001144929.1
• UniProt Accession #: Q9NYR9; Q6PK52; Q96KC7; Q9NSX1; A6NCZ5; B3KNN0; B4DNM3
• Molecular Weight: 21,508 Da
• NCBI Official Full Name: NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform c
• NCBI Official Synonym Full Names: NFKB inhibitor interacting Ras-like 2
• NCBI Official Symbol: NKIRAS2
• NCBI Official Synonym Symbols: KBRAS2; kappaB-Ras2
• NCBI Protein Information: NF-kappa-B inhibitor-interacting Ras-like protein 2; kappa B-Ras protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2
• UniProt Protein Name: NF-kappa-B inhibitor-interacting Ras-like protein 2
• Protein Family: NF-kappa-B inhibitor-interacting Ras-like protein
• UniProt Gene Name: NKIRAS2
• UniProt Synonym Gene Names: KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2
• UniProt Entry Name: KBRS2_HUMAN

Uniprot Description

NKIRAS2: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: membrane; cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction