Mouse Neurofilament H, 200kD Monoclonal Antibody

Neurofilament H, 200kD (NF-H)

Cat. Num. AAA631852

• Reactivity: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Neurofilament H; 200kD; Monoclonal Antibody; 200kD (NF-H); Anti -Neurofilament H; anti-Neurofilament H; 200kD antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 4A75
• Specificity: Reacts with the intermediate filament protein of central and peripheral nerve cells. In Western blotting reactive only with the 200kD neurofilament protein. Reacts with both the phosphorylated and non-phosphorylated isoform. Species crossreactivity: Human, rat, rabbit, guinea pig, canine, hamster, chicken, mouse, bovine, monkey, sheep, Xenopus.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.

• Applicable Applications for anti-Neurofilament H, 200kD antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry (frozen and paraffin sections) and Western Blotting., Optimum dilutions to be determined by researcher.
• Immunogen: Neurofilament, 200kD
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Neurofilament H, 200kD antibody

Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200kD) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70kD NF-protein can self-assemble into a filamentous structure, whereas the 160kD and 200kD NF-proteins require the presence of the 70kD NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. The 200kD neurofilament protein is abundantly expressed in the spinal cord, cerebellum, pons and medulla. These are predominantly brain regions giving rise to long-axon neurons.

Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes are NF proteins expressed together with desmin. In tumorpathology ganglioneuro-blastomas and some of the neuroblastomas are strongly positive with the neurofilament antisera. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation [1].

Product Categories/Family for anti-Neurofilament H, 200kD antibody

Antibodies; Abs to Neurofilament