Related Product Information for anti-Neurofilament H, 200kD antibody
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200kD) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70kD NF-protein can self-assemble into a filamentous structure, whereas the 160kD and 200kD NF-proteins require the presence of the 70kD NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. The 200kD neurofilament protein is abundantly expressed in the spinal cord, cerebellum, pons and medulla. These are predominantly brain regions giving rise to long-axon neurons.
Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes are NF proteins expressed together with desmin. In tumorpathology ganglioneuro-blastomas and some of the neuroblastomas are strongly positive with the neurofilament antisera. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.
Product Categories/Family for anti-Neurofilament H, 200kD antibody
Product Notes
The Neurofilament H, 200kD (Catalog #
AAA609383) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament H, 200kD (NF-H) reacts with Bovine, Canine, Chicken, Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament H, 200kD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Immunoblot and Immunohistochemistry. Dilution: Immunoblot: 1:50-1:250 Immunohistochemistry (frozen tissues): 1:25-1:50. Recommended Detecting Agent: Avidin-biotinylated horseradish peroxidase complex (ABC). Optimum dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Neurofilament H, 200kD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"Neurofilament H, 200kD, Monoclonal Antibody" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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