Human PRODH (Proline dehydrogenase 1, mitochondrial) ELISA Kit | PRODH elisa kit

Human PRODH (Proline dehydrogenase 1, mitochondrial) ELISA Kit

Cat. Num. AAA7606711

• Gene Names: PRODH; POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6
• Reactivity: Human
• Synonyms: PRODH (Proline dehydrogenase 1; mitochondrial); Human PRODH (Proline dehydrogenase 1; mitochondrial) ELISA Kit; PRODH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRODH. No significant cross-reactivity or interference between PRODH and analogues was observed.
• Sequence Length: 600

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRODH elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 304766736
• NCBI GeneID: 5625
• NCBI Accession #: NP_057419.4
• NCBI GenBank Nucleotide #: NM_016335.4
• UniProt Accession #: O43272; O14680; Q0P507; Q147W8; Q504W1; Q59FI8; Q6NV86; Q9UF13; A6NF53
• Molecular Weight: 59,231 Da
• NCBI Official Full Name: proline dehydrogenase 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: proline dehydrogenase 1
• NCBI Official Symbol: PRODH
• NCBI Official Synonym Symbols: POX; PIG6; HSPOX2; PRODH1; PRODH2; TP53I6
• NCBI Protein Information: proline dehydrogenase 1, mitochondrial
• UniProt Protein Name: Proline dehydrogenase 1, mitochondrial
• UniProt Gene Name: PRODH

NCBI Description

This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

PRODH: Converts proline to delta-1-pyrroline-5-carboxylate. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1). HP-1 is a disorder characterized by elevated serum proline levels. May be involved in the psychiatric and behavioral phenotypes associated with the 22q11 velocardiofacial and DiGeorge syndrome. Defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. Belongs to the proline oxidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.5.5.2; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: mitochondrial inner membrane; mitochondrion

Molecular Function: proline dehydrogenase activity

Biological Process: 4-hydroxyproline catabolic process; proline catabolic process; proline catabolic process to glutamate; proline metabolic process

Disease: Hyperprolinemia, Type I; Schizophrenia 4