Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast carcinoma stained with Neu antibody using peroxidase-conjugate and AEC chromogen. Note intense cell membrane staining of tumor cells.)
Mouse Neu Monoclonal Antibody
Neu antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Neu antibody was purified by Protein A chromatography.
Synonyms
Neu; Monoclonal Antibody; Neu antibody; Monoclonal Neu; Anti-Neu; anti-Neu antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
e2-4001
Purity/Purification
Neu antibody was purified by Protein A chromatography.
Form/Format
Antibody purified from ascites fluid by protein G chromotography and supplied in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% NaN3.
Concentration
0.2 mg/ml (varies by lot)
Applicable Applications for anti-Neu antibody
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IHC: 1:200-1:400
IP: 2 ug/mg protein lysate
WB: 1-2 ug/ml
IP: 2 ug/mg protein lysate
WB: 1-2 ug/ml
Biological Significance
c-erbB-2, second member (c-erbB-2/HER-2/neu) of the c-erbB family is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein.
Cross-Reactivity
Human, mouse, rat
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
Immunogen
Neu antibody was raised in mouse using cytoplasmic domain of recombinant human c-erbB-2/HER-2 as the immunogen.
Preparation and Storage
Store at 4 degree C.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast carcinoma stained with Neu antibody using peroxidase-conjugate and AEC chromogen. Note intense cell membrane staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast carcinoma stained with Neu antibody using peroxidase-conjugate and AEC chromogen. Note intense cell membrane staining of tumor cells.)
Related Product Information for anti-Neu antibody
Mouse monoclonal Neu antibody
Product Categories/Family for anti-Neu antibody
Similar Products
Product Notes
The Neu (Catalog #AAA530119) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neu antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neu can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IHC: 1:200-1:400 IP: 2 ug/mg protein lysate WB: 1-2 ug/ml. Researchers should empirically determine the suitability of the Neu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neu, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
