Dot Blot (DB)
(Dot Blot analysis on nitrocellulose membrane using MBS6004923 (0.6ug/ml). 50ng of Phospho-peptide (bottom) or Non Phospho-peptide (top) per dot were adsorbed.)
Rabbit anti-Human Sox2 Polyclonal Antibody | anti-Sox2 antibody
Sox2, Phosphorylated (S246) (SOX2, Transcription factor SOX-2) (MaxLight 750)
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human
Applications
Immunofluorescence, Dot Blot, ELISA
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
Sox2; Polyclonal Antibody; Phosphorylated (S246) (SOX2; Transcription factor SOX-2) (MaxLight 750); SOX2; Transcription factor SOX-2; anti-Sox2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Sox2.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-Sox2 antibody
Immunofluorescence (IF), Dot Blot (DB), ELISA (EIA)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic phospho-peptide mapping to a fragment of residues surrounding S246 of human Sox2, UniProt Accession #P48431. Species sequence homology: mouse
Conjugate
MaxLight750
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Dot Blot (DB)
(Dot Blot analysis on nitrocellulose membrane using MBS6004923 (0.6ug/ml). 50ng of Phospho-peptide (bottom) or Non Phospho-peptide (top) per dot were adsorbed.)
Dot Blot (DB)
(Dot Blot analysis on nitrocellulose membrane using MBS6004923 (0.6ug/ml). 50ng of Phospho-peptide (bottom) or Non Phospho-peptide (top) per dot were adsorbed.)
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells usingMBS6004923. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS6004923 (1:200, 2h at RT). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.)
Immunofluorescence (IF)
(Fluorescent confocal image of SY5Y cells usingMBS6004923. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS6004923 (1:200, 2h at RT). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.)
Related Product Information for anti-Sox2 antibody
MaxLight750 is a new Near IR stable dye conjugate comparable to DyLight750, Alexa Fluor700 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
Product Categories/Family for anti-Sox2 antibody
References
1. Gen, Y., et al. Cancer Genet. Cytogenet. 202(2):82-93(2010). 2. Ji, J., et al. Hum. Pathol. 41(10):1438-1447(2010). 3. Fang, X., et al. OMICS 14(4):369-384(2010). 4. Sholl, L.M., et al. Am. J. Surg. Pathol. 34(8):1193-1198(2010). 5. Zhang, D., et al. BMC Med. Genet. 11, 116 (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34kDa
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY (sex determining region Y)-box 2
NCBI Official Symbol
SOX2
NCBI Official Synonym Symbols
ANOP3; MCOPS3
NCBI Protein Information
transcription factor SOX-2; transcription factor SOX2; SRY-related HMG-box gene 2
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
SOX2
UniProt Entry Name
SOX2_HUMAN
Similar Products
Product Notes
The Sox2 sox2 (Catalog #AAA6350006) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sox2, Phosphorylated (S246) (SOX2, Transcription factor SOX-2) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sox2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Dot Blot (DB), ELISA (EIA). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Sox2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sox2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
