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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS968919 at dilution 1:100)

Rabbit anti-Human TCF12 Polyclonal Antibody | anti-TCF12 antibody

TCF12 Antibody

Gene Names
TCF12; HEB; CRS3; HTF4; TCF-12; bHLHb20; HsT17266
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
TCF12; Polyclonal Antibody; TCF12 Antibody; TCF-12; Class B basic helix-loop-helix protein 20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4; BHLHB20; HEB; HTF4; anti-TCF12 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
536
Applicable Applications for anti-TCF12 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
Recommended dilution: IHC:1:20-1:200
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Transcription factor 12 protein (443-682aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-23128 / sc-23127 / sc-357 / sc-22826
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain tissue using MBS968919 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS968919 at dilution 1:100)

Immunoprecipitation (IP)

(Immunoprecipitating TCF12 in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS968919 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS968919(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))

Immunoprecipitation (IP) (Immunoprecipitating TCF12 in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS968919 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS968919(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))
Related Product Information for anti-TCF12 antibody
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,157 Da
NCBI Official Full Name
transcription factor 12 isoform d
NCBI Official Synonym Full Names
transcription factor 12
NCBI Official Symbol
TCF12
NCBI Official Synonym Symbols
HEB; CRS3; HTF4; TCF-12; bHLHb20; HsT17266
NCBI Protein Information
transcription factor 12
UniProt Protein Name
Transcription factor 12
Protein Family
UniProt Gene Name
TCF12
UniProt Synonym Gene Names
BHLHB20; HEB; HTF4; TCF-12; bHLHb20
UniProt Entry Name
HTF4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').

Research Articles on TCF12

Similar Products

Product Notes

The TCF12 tcf12 (Catalog #AAA968919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF12 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCF12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP). Recommended dilution: IHC:1:20-1:200. Researchers should empirically determine the suitability of the TCF12 tcf12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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