NKp30/NCR3 Recombinant Protein | NCR3 recombinant protein

NKp30/NCR3 Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8120303

• Gene Names: NCR3; 1C7; MALS; CD337; LY117; NKp30
• Purity: >95% as determined by SDS-PAGE
• Synonyms: NKp30/NCR3; NKp30/NCR3 Protein; Human; Recombinant (ECD; hFc Tag); Human NCR3 (CD337) Protein (Fc Tag); 1C7 Protein; CD337 Protein; DAAP-90L16.3 Protein; LY117 Protein; MALS Protein; NCR3 Protein; NKp30 Protein; natural cytotoxicity triggering receptor 3; NCR3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly135

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 19
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human NCR3 (NP_001138938.1)(Met1-Gly135) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NCR3 recombinant protein

Background: Natural Cytotoxicity Triggering Receptor 3, NCR3, also known as NKp30, or CD337, is a natural cytotoxicity receptor, expressed on subsets of human peripheral blood NK cells, involved in NK cell killing of tumor cells and immature dendritic cells. The cellular ligand for NKp30 has remained elusive, but the membrane-associated heparan sulfate (HS) proteoglycans are involved in the recognition of cellular targets by NKp30 was recently reported. NKp30 is a member of the immunoglobulin superfamily and one of three existing natural cytotoxicity-triggering receptors. NKp30 is a glycosylated protein and is thought to be selectively expressed in resting and activated natural killer cells. NKp30 is a stimulatory receptor on human NK cells implicated in tumor immunity and is capable of promoting or terminating dendritic cell maturation. NCR3 may play a role in inflammatory and infectious diseases.

Product Categories/Family for NCR3 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Warren HS, et al. (2005) Evidence that the cellular ligand for the human NK cell activation receptor NKp30 is not a heparan sulfate glycosaminoglycan. J Immunol. 175(1): 207-12.Mulcahy H, et al. (2006) LST1 and NCR3 expression in autoimmune inflammation and in response to IFN-gamma, LPS and microbial infection. Immunogenetics. 57(12): 893-903.Hsieh CL, et al. (2006) NKp30 is a functional activation receptor on a subset of rat natural killer cells. Eur J Immunol. 36(8): 2170-80.Ponnampalam AP, et al. (2008) Identification and hormonal regulation of a novel form of NKp30 in human endometrial epithelium. Eur J Immunol. 38(1): 216-26.

NCBI and Uniprot Product Information

• NCBI GI #: 224586860
• NCBI GeneID: 259197
• NCBI Accession #: NP_001138938.1
• NCBI GenBank Nucleotide #: NM_001145466.1
• UniProt Accession #: O14931; O14930; O14932; O95667; O95668; O95669; Q5ST89; Q5ST90; Q5ST91; B0S8F2; B0S8F4; B0S8F5
• Molecular Weight: 21,593 Da
• NCBI Official Full Name: natural cytotoxicity triggering receptor 3 isoform b
• NCBI Official Synonym Full Names: natural cytotoxicity triggering receptor 3
• NCBI Official Symbol: NCR3
• NCBI Official Synonym Symbols: 1C7; MALS; CD337; LY117; NKp30
• NCBI Protein Information: natural cytotoxicity triggering receptor 3; NK-p30; lymphocyte antigen 117; activating NK-A1 receptor; activating natural killer receptor p30; natural killer cell p30-related protein
• UniProt Protein Name: Natural cytotoxicity triggering receptor 3
• Protein Family: Natural cytotoxicity triggering receptor
• UniProt Gene Name: NCR3
• UniProt Synonym Gene Names: 1C7; LY117; NK-p30; NKp30
• UniProt Entry Name: NCTR3_HUMAN

NCBI Description

The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

Uniprot Description

NCR3: Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation. Belongs to the natural cytotoxicity receptor (NCR) family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: integral to plasma membrane

Biological Process: immune response; cell recognition; inflammatory response; positive regulation of natural killer cell mediated cytotoxicity

Disease: Malaria, Mild, Susceptibility To