Western Blot (WB)
(Western blot without neuraminidase treatment (Lane 1) and with neuraminidase treatment (Lane 2). Treated protein shows major bands at 180kD and 140kD, with minor degradation band at 160kD.)
CD56 Protein
CD56 (NCAM-1, Neural Cell Adhesion Molecule)
Purity
Purified. When treated with Neurminidase, molecular weight is 140-180kD. A breakdown product of 160kD may also be visible.
Synonyms
CD56; CD56 (NCAM-1; Neural Cell Adhesion Molecule); CD56 protein
Host
Embryonic chicken brain
Purity/Purification
Purified. When treated with Neurminidase, molecular weight is 140-180kD. A breakdown product of 160kD may also be visible.
Form/Format
Supplied as a liquid in 15mM octylthioglucoside, sodium bicarbonate. No preservative added.
Concentration
0.5 mg/ml (varies by lot)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot without neuraminidase treatment (Lane 1) and with neuraminidase treatment (Lane 2). Treated protein shows major bands at 180kD and 140kD, with minor degradation band at 160kD.)
Western Blot (WB)
(Western blot without neuraminidase treatment (Lane 1) and with neuraminidase treatment (Lane 2). Treated protein shows major bands at 180kD and 140kD, with minor degradation band at 160kD.)
Related Product Information for CD56 protein
The neural cell adhesion molecule (NCAM) was the first cell-cell adhesion molecule to be identified, isolated, and sequenced. Structurally, the extracellular portion of NCAM is comprised of five IgG-like domains followed by two fibronectin type III repeats. Because of its history, NCAM is considered to be the classic example of the family of cell-cell adhesion molecules that are structurally related to immunoglobulins. There are three forms of NCAM molecules that differ in their mode of attachment to the plasma membrane: ld, transmembrane form with large cytoplasmic domain; sd,transmembrane form with small cytoplasmic domain; ssd, GPI linked to the external surface of the plasma membrane. NCAM is unique in that the molecule can bear large oligosaccharides containing long chains of polysialic acid.
Functionally, NCAM mediates cell-cell adhesion both in neural cells and a variety of other cell types. NCAM-mediated adhesion occurs by both a homophilic mechanism (NCAM to NCAM) and a heterophilic mechanism in which NCAM binds to heparin sulfate proteoglycans. The polysialic acid in NCAM interferes with NCAMmediated cell-cell adhesion. Moreover, the polysialic acid on NCAM fills up so much of the space between cells that it also interferes with cell-cell adhesion mediated by other adhesion molecules. It has been suggested that the polysialic acid on NCAM is involved in maintaining plasticity in interactions among neural cells. NCAM is expressed at a wide variety of sites during embryonic development both in the nervous system and other tissues. Its expression is more restricted in the adult brain; however, it is still expressed in certain regions. NCAM expression is also associated with certain diseases. In particular, it is considered to be a marker for Wilms tumor and for small cell lung carcinoma. NCAM is derived from embryonic chicken brains and is composed of polysialic acid-rich molecules. Neuraminidase treatment of the preparation reveals that it contains mainly the ld form of NCAM with a smaller amount of the sd form.
Functionally, NCAM mediates cell-cell adhesion both in neural cells and a variety of other cell types. NCAM-mediated adhesion occurs by both a homophilic mechanism (NCAM to NCAM) and a heterophilic mechanism in which NCAM binds to heparin sulfate proteoglycans. The polysialic acid in NCAM interferes with NCAMmediated cell-cell adhesion. Moreover, the polysialic acid on NCAM fills up so much of the space between cells that it also interferes with cell-cell adhesion mediated by other adhesion molecules. It has been suggested that the polysialic acid on NCAM is involved in maintaining plasticity in interactions among neural cells. NCAM is expressed at a wide variety of sites during embryonic development both in the nervous system and other tissues. Its expression is more restricted in the adult brain; however, it is still expressed in certain regions. NCAM expression is also associated with certain diseases. In particular, it is considered to be a marker for Wilms tumor and for small cell lung carcinoma. NCAM is derived from embryonic chicken brains and is composed of polysialic acid-rich molecules. Neuraminidase treatment of the preparation reveals that it contains mainly the ld form of NCAM with a smaller amount of the sd form.
Product Categories/Family for CD56 protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CD56 (Catalog #AAA634928) is a Protein produced from Embryonic chicken brain and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD56, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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