Testing Data
(Figure 1: Immunostaining of the neural cell adhesion molecule (NCAM) in small cell lung cancer cell line NCI-H82
)
Mouse NCAM / CD56 Monoclonal Antibody
Mouse anti NCAM / CD56
Reactivity
Human, Zebrafish
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
NCAM / CD56; Monoclonal Antibody; Mouse anti NCAM / CD56; anti-NCAM / CD56 antibody
Host
Mouse
Reactivity
Human, Zebrafish
Clonality
Monoclonal
Isotype
IgG1
Clone Number
123C3
Specificity
123C3 was defined as a cluster I antibody during the First International Workshop on Small Cell Lung Cancer (SCLC) Antibodies. 123C3 recognizes an epitope in the NCAM exons 11-13 which is dependent on an intact conformation of the first fibronectin type-III homologous domain encoded by these exons. 123C3 stains NCAM which is present in small cell lung cancer and lung carcinoids. It also reacts with a number of non-small cell lung carcinomas and neuroendocrine and neuronal derived tissues. In addition, 123C3 is internalized after binding to its antigen on SCLC cell lines, making it an excellent reagent for tumor imaging in xenograft models.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-NCAM / CD56 antibody
Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
123C3 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
123C3 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with the a membrane prepaRation of a small cell lung carcinoma specimen.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunostaining of the neural cell adhesion molecule (NCAM) in small cell lung cancer cell line NCI-H82 )
Testing Data
(Figure 1: Immunostaining of the neural cell adhesion molecule (NCAM) in small cell lung cancer cell line NCI-H82
)
Testing Data
(Figure 2: Immunofluorescence staining of a 9 days old zebrafish embryo )
Testing Data
(Figure 2: Immunofluorescence staining of a 9 days old zebrafish embryo
)
Related Product Information for anti-NCAM / CD56 antibody
NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be geneRated by alternative splicing and by posttranslational modifiCations, such as sialylation. During sialylation, polysialic acid (PSA) carbohydRates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.
Product Categories/Family for anti-NCAM / CD56 antibody
References
Schol, D. J., Mooi, W. J., van der Gugten, A. A., Wagenaar, S. S., and Hilgers, J. (1988). Monoclonal antibody 123C3, identifying small cell carcinoma phenotype in lung tumours, recognizes mainly, but not exclusively, endocrine and neuron-supporting normal tissues, Int J Cancer Suppl 2, 34-40. Mooi, W. J., Wagenaar, S. S., Schol, D., and Hilgers, J. (1988). Monoclonal antibody 123C3 in lung tumour classifiCation. Immunohistology of 358 resected lung tumours, Mol Cell Probes 2, 31-7. Moolenaar, C. E., Muller, E. J., Schol, D. J., Figdor, C. G., Bock, E., Bitter-Suermann, D., and Michalides, R. J. (1990). Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212, Cancer Res 50, 1102-6. Kibbelaar, R. E., Moolenaar, K. E., Michalides, R. J., Van Bodegom, P. C., Vanderschueren, R. G., Wagenaar, S. S., Dingemans, K. P., Bitter-Suermann, D., Dalesio, O., Van Zandwijk, N., and et al. (1991). Neural cell adhesion molecule expression, neuroendocrine differentiation and prognosis in lung carcinoma, Eur J Cancer 27, 431-5. Gerardy-Schahn, R., and Eckhardt, M. (1994). Hot spots of antigenicity in the neural cell adhesion molecule NCAM, Int J Cancer Suppl 8, 38-42. Kwa, H. B., Verhoeven, A. H., Storm, J., van Zandwijk, N., Mooi, W. J., and Hilkens, J. (1995). Radioimmunotherapy of small-cell lung cancer xenografts using 131I- labelled anti-NCAM monoclonal antibody 123C3, Cancer Immunol Immunother 41, 169-74. Kwa, H. B., Verheijen, M. G., Litvinov, S. V., Dijkman, J. H., Mooi, W. J., and Van Krieken, J. H. (1996). Prognostic factors in resected non-small cell lung cancer: an immunohistochemical study of 39 cases, Lung Cancer 16, 35-45.
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Product Notes
The NCAM / CD56 (Catalog #AAA570110) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti NCAM / CD56 reacts with Human, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's NCAM / CD56 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). 123C3 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the NCAM / CD56 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCAM / CD56, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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