Mouse N-Cadherin/Cadherin-2/CD325 (NCAD) Monoclonal Antibody | anti-NCAD antibody

N-Cadherin/Cadherin-2/CD325 (NCAD)

Cat. Num. AAA438959

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Human, Mouse. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: N-Cadherin/Cadherin-2/CD325 (NCAD); Monoclonal Antibody; Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD; anti-NCAD antibody

• Host: Mouse
• Reactivity: Human, Mouse. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 8C11
• Specificity: Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 875

• Applicable Applications for anti-NCAD antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; WB: 0.5-1.0ug/ml; IHC-F: 0.5-1.0ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tis with1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant human N-cadherin extracellular domain
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 18q11.2
• Positive Control: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Wahl, JK III; Kim, YJ; Cullen, JM; Johnson, KR; Wheelock, MJ. N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem. 2003, 278(19):17269-7.

NCBI and Uniprot Product Information

• NCBI GI #: 815890961
• NCBI GeneID: 1000
• NCBI Accession #: NP_001295105.1
• NCBI GenBank Nucleotide #: NM_001308176.1
• UniProt Accession #: P19022; Q14923; Q8N173; A8MWK3; B0YIY6
• Molecular Weight: 130-140kDa
• NCBI Official Full Name: cadherin-2 isoform 2
• NCBI Official Synonym Full Names: cadherin 2
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2
• UniProt Protein Name: Cadherin-2
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin

NCBI Description

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density ().