Western Blot (WB)
(Western blot analysis of extracts of HeLacell line, using LMNB1 antibody.)
Rabbit LMNB1 Polyclonal Antibody | anti-LMNB1 antibody
LMNB1 Polyclonal Antibody
Gene Names
LMNB1; LMN; ADLD; LMN2; LMNB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Synonyms
LMNB1; Polyclonal Antibody; LMNB1 Polyclonal Antibody; ADLD; LMN; LMN2; LMNB; MGC111419; Lamin B1; anti-LMNB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
586
Applicable Applications for anti-LMNB1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:20-1:50
IP: 1:20-1:50
IHC: 1:50-1:100
IF: 1:20-1:50
IP: 1:20-1:50
Immunogen
Fusion protein of human LMNB1
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of HeLacell line, using LMNB1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLacell line, using LMNB1 antibody.)
Related Product Information for anti-LMNB1 antibody
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, arising from alternative splicing. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4, 5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene is correlated with the pathogenesis of the neurological disorder adult-onset leukodystrophy (10).
References
1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23. 2. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93. 3. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84. 4. Rao, L. et al. (1996) J Cell Biol 135, 1441-55. 5. Orth, K. et al. (1996) J Biol Chem 271, 16443-6. 6. Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506. 7. Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6. 8. Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75. 9. Chandler, J.M. et al. (1997) Biochem J 322 (Pt 1), 19-23. 10. Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66kDa
NCBI Official Full Name
lamin-B1 isoform 1
NCBI Official Synonym Full Names
lamin B1
NCBI Official Symbol
LMNB1
NCBI Official Synonym Symbols
LMN; ADLD; LMN2; LMNB
NCBI Protein Information
lamin-B1
UniProt Protein Name
Lamin-B1
Protein Family
UniProt Gene Name
LMNB1
UniProt Synonym Gene Names
LMN2; LMNB
NCBI Description
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Uniprot Description
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Research Articles on LMNB1
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Product Notes
The LMNB1 lmnb1 (Catalog #AAA8527397) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LMNB1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LMNB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:20-1:50 IP: 1:20-1:50. Researchers should empirically determine the suitability of the LMNB1 lmnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMNB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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