Cadherin-2/N-Cadherin ELISA Kit | CDH2 elisa kit

Monkey primate Cadherin-2/N-Cadherin PicoKine ELISA Kit

Cat. Num. AAA1751070

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: Cadherin-2/N-Cadherin; Monkey primate Cadherin-2/N-Cadherin PicoKine ELISA Kit; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD325; CDH2; CDHN; NCAD; CDH2 elisa kit

• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant 215 Monkey primate Cadherin-2/N-Cadherin
• Sequence Length: 875

• Applicable Applications for CDH2 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 0.78ng/ml-50ng/ml
• Sensitivity: <20pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDH2 elisa kit

Principle of the Assay: 215 Monkey primate Cadherin-2/N-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey primate Cadherin-2/N-Cadherin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey primate Cadherin-2/N-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey primate Cadherin-2/N-Cadherin amount of sample captured in plate.

Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Product Categories/Family for CDH2 elisa kit

Signal Transduction; Cytoskeleton/ECM; Cell Adhesion; cadherins

NCBI and Uniprot Product Information

• NCBI GI #: 815890961
• NCBI GeneID: 1000
• NCBI Accession #: NP_001295105.1
• NCBI GenBank Nucleotide #: NM_001308176.1
• UniProt Accession #: P19022; Q14923; Q8N173; A8MWK3; B0YIY6
• Molecular Weight: 99809 MW
• NCBI Official Full Name: cadherin-2 isoform 2
• NCBI Official Synonym Full Names: cadherin 2
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin

NCBI Description

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density ().