Mouse anti-Human Myoglobin Monoclonal Antibody | anti-MB antibody

Myoglobin

Cat. Num. AAA201210

• Gene Names: MB; PVALB
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: By protein-A affinity chromatography
• Synonyms: Myoglobin; Monoclonal Antibody; PVALB; anti-MB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: AT6E10
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 154

• Applicable Applications for anti-MB antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity.
• Antigen Species: Human
• Immunogen: Recombinant human Myoglobin(1-154aa) purified from E. coli
• Conjugation: Unconjugated
• Note: This product was produced from tissue culture supernatant.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myoglobin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mosue muscle tissue lysate Lane 2.: Mouse heart tissue lysate )

Related Product Information for anti-MB antibody

MB (Myoglobin) is an iron- and oxygen-binding protein. It is found in abundance in the muscle tissue of vertebrates in general and in almost all mammals. MB (myglobin) is related to hemoglobin, which consists of four myoglobin-like subunits that form a tetramer and are responsible for carrying oxygen in blood. The only time myoglobin is found in the bloodstream is when it is released following muscle injury. It is an abnormal finding, and can be identificationally relevant when found in blood.

Product Categories/Family for anti-MB antibody

Cancer; Cardiovascular; Development; Epigenetics and Nuclear Signaling; Immunology; Lysates; Metabolism; Neuroscience; Other; Signal Transduction; Stem cell; Tags & Cell Markers

References

George A., et al. (2004). Journal of Experimental Biology 207 (Pt 20): 3441-6.; Akaboshi E (1985). Gene 33 (3): 241-9.; J. P. Collman., et al. (2004). Chem. Rev. 104 (2): 561-588.

NCBI and Uniprot Product Information

• NCBI GI #: 44955888
• NCBI GeneID: 4151
• NCBI Accession #: NP_976312
• NCBI GenBank Nucleotide #: NP_976312.1
• UniProt Accession #: P02144
• NCBI Official Full Name: myoglobin isoform 1
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• Protein Family: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN

NCBI Description

This gene encodes a member of the globin superfamily and is predominantly expressed in skeletal and cardiac muscles. The encoded protein forms a monomeric globular haemoprotein that is primarily responsible for the storage and facilitated transfer of oxygen from the cell membrane to the mitochondria. This protein also plays a role in regulating physiological levels of nitric oxide. Multiple transcript variants encoding distinct isoforms exist for this gene. [provided by RefSeq, May 2020]

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hormone stimulus; response to hypoxia; brown fat cell differentiation; enucleate erythrocyte differentiation