Western Blot (WB)
(The extracts of mouse muscle and heart (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human, Mouse MB Monoclonal Antibody | anti-MB antibody
MB antibody
Gene Names
MB; PVALB; myoglobgin
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
By protein-G affinity chromatography
Synonyms
MB; Monoclonal Antibody; MB antibody; Myoglobin; PVALB; anti-MB antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT6E10
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
154
Applicable Applications for anti-MB antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:1000.
Antigen Species
Human
Immunogen
Recombinant human MB (1-154aa) purified from E. coli
Clone Note
Anti-human MB mAb, clone AT6E10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MB protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The extracts of mouse muscle and heart (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(The extracts of mouse muscle and heart (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-MB antibody
MB (Myoglobin) is an an iron- and oxygen-binding protein. It is found in abundance in the muscle tissue of vertebrates in general and in almost all mammals. MB (myglobin) is related to hemoglobin, which consists of four myoglobin-like subunits that form a tetramer and are responsible for carrying oxygen in blood. The only time myoglobin is found in the bloodstream is when it is released following muscle injury. It is an abnormal finding, and can be relevant when found in blood.
Product Categories/Family for anti-MB antibody
References
George A., et al. (2004). Journal of Experimental Biology 207 (Pt 20): 3441-6.; Akaboshi E (1985). Gene 33 (3): 241-9.; J. P. Collman., et al. (2004). Chem. Rev. 104 (2): 561-588.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,184 Da
NCBI Official Full Name
myoglobin
NCBI Official Synonym Full Names
myoglobin
NCBI Official Symbol
MB
NCBI Official Synonym Symbols
PVALB; myoglobgin
NCBI Protein Information
myoglobin
UniProt Protein Name
Myoglobin
Protein Family
UniProt Gene Name
MB
NCBI Description
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Research Articles on MB
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Product Notes
The MB mb (Catalog #AAA200198) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MB antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the MB mb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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