Western Blot (WB)
(Figure 1. Western blot analysis using Myoglobin mouse mAb against truncated Myoglobin recombinant protein.)
Mouse anti-Human Myoglobin Antibody | anti-MB antibody
Myoglobin Antibody
Gene Names
MB; PVALB; myoglobgin
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
Myoglobin; Myoglobin Antibody; PVALB; MGC13548; anti-MB antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
6H8B5; 5A2G8; 4D7H3; 3H6A7
Form/Format
Purified antibody in PBS containing 0.03% sodium azide.
Sequence Length
154
Applicable Applications for anti-MB antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000
IHC: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of Myoglobin expressed in E. Coli
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Western Blot (WB)
(Figure 1. Western blot analysis using Myoglobin mouse mAb against truncated Myoglobin recombinant protein.)
Western Blot (WB)
(Figure 1. Western blot analysis using Myoglobin mouse mAb against truncated Myoglobin recombinant protein.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue showing cytoplasmic localization using anti-Myoglobin antibody with DAB staining.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue showing cytoplasmic localization using anti-Myoglobin antibody with DAB staining.)
Related Product Information for anti-MB antibody
Myoglobin (MB), with 154-amino acid protein (about 17kDa), is a member of the globin superfamily and expression of myoglobin is highest in skeletal and cardiac muscle. Functionally, myoglobin is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O2 concentration when muscle activity increases and to facilitate intracellular O2 diffusion by providing a parallel path that augments simple diffusion of dissolved O2. Furthermore, myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI identification.
Product Categories/Family for anti-MB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,184 Da
NCBI Official Full Name
myoglobin
NCBI Official Synonym Full Names
myoglobin
NCBI Official Symbol
MB
NCBI Official Synonym Symbols
PVALB; myoglobgin
NCBI Protein Information
myoglobin
UniProt Protein Name
Myoglobin
Protein Family
UniProt Gene Name
MB
NCBI Description
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Research Articles on MB
Similar Products
Product Notes
The MB mb (Catalog #AAA850910) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Myoglobin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myoglobin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the MB mb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myoglobin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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