Rabbit MAP1LC3 Polyclonal Antibody | anti-MAP1LC3A antibody

MAP1LC3 Antibody

Cat. Num. AAA153466

• Gene Names: MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: MAP1LC3 antibody is affinity chromatography purified via peptide column.
• Synonyms: MAP1LC3; Polyclonal Antibody; MAP1LC3 Antibody; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3Autophagy-related protein LC3 A; microtubule-associated protein 1 light chain 3 alpha; anti-MAP1LC3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAP1LC3 antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3 are known to exist. MAP1LC3 antibody is predicted to detect MAP1LC3A, MAP1LC3B, and MAP1LC3C.
• Purity/Purification: MAP1LC3 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 121

• Applicable Applications for anti-MAP1LC3A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: MAP1LC3 antibody can be used for detection of MAP1LC3 by Western blot at 0.5 - 1 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: MAP1LC3 antibody was raised against a 12 amino acid peptide near the center of human MAP1LC3A.
• Buffer: MAP1LC3 antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: MAP1LC3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of MAP1LC3 in human brain tissue lysate with MAP1LC3 antibody at (A) 1 and (B) 2 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of MAP1LC3 in rat brain tissue with MAP1LC3 antibody at 5 μg/ml.)

Related Product Information for anti-MAP1LC3A antibody

Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3 is a subfamily of three related proteins belonging to the MAP1 LC3 family and it includes MAP1LC3A, MAP1LC3B, and MAP1LC3C (2). MAP1LC3 is the mammalian homolog of yeast ATG8 and is essential for autophagy and associated with the autophagosome membranes after processing (3). The three isoforms exhibit distinct expression patterns and both MAP1LC3A and MAP1LC3B but not MAP1LC3B, are post-translationally modified, suggesting the three isoforms may have different physiological functions (4).

NCBI and Uniprot Product Information

• NCBI GI #: 14210522
• NCBI GeneID: 84557
• NCBI Accession #: NP_115903
• NCBI GenBank Nucleotide #: NM_032514.3
• UniProt Accession #: Q9H492; Q9BXW5; E1P5P4; E1P5P5
• Molecular Weight: Predicted: 13 kDa; ; Observed: 18 kDa
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3A isoform a
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 alpha
• NCBI Official Symbol: MAP1LC3A
• NCBI Official Synonym Symbols: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3A; MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1A/MAP1B light chain 3 A; MAP1 light chain 3-like protein 1; autophagy-related ubiquitin-like modifier LC3 A
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3A
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3A
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 A
• UniProt Entry Name: MLP3A_HUMAN

NCBI Description

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Uniprot Description

LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Vesicle; Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding

Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation