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Immunohistochemistry (IHC)
(Immunofluorescence staining of muscle tissue of 7 days old zebrafish embryo. Note reactivity in both striated and smooth muscle tissues Upper panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining)
Mouse Muscle Actin Monoclonal Antibody | anti-IgG1 antibody
Anti-Muscle Actin Antibody (clone HHF35) IHC-plus
Reactivity
Chicken, Zebrafish, Pig, Rabbit, Rat, Human, Monkey
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Purified
Synonyms
Muscle Actin; Monoclonal Antibody; Anti-Muscle Actin Antibody (clone HHF35) IHC-plus; Mouse Monoclonal [clone HHF35] (IgG1) to Human Muscle Actin; Human Muscle Actin; anti-IgG1 antibody
Host
Mouse
Reactivity
Chicken, Zebrafish, Pig, Rabbit, Rat, Human, Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HHF35
Specificity
Reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections. The epitope recognized by HHF35 is highly conserved. Therefore the antibody cross-reacts with several species including human, monkey, swine, rabbit, rat, chicken and zebrafish.
Purity/Purification
Purified
Form/Format
PBS containing 0.09% sodium azide
Sequence Length
376
Applicable Applications for anti-IgG1 antibody
Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB), ELISA (EIA), Electron Microscopy
Application Notes
IHC-Fr (1:200 - 1:1000)
IHC-P (5 ug/ml)
WB (1:1000 - 1:5000)
IHC-P (5 ug/ml)
WB (1:1000 - 1:5000)
Immunogen
Muscle Actin antibody was raised against sDS-extracted protein fraction from human myocardium
Immunogen Description
SDS-extracted protein fraction from human myocardium
Target Species
Human
Preparation and Storage
4 degree C, avoid freezing
Immunohistochemistry (IHC)
(Immunofluorescence staining of muscle tissue of 7 days old zebrafish embryo. Note reactivity in both striated and smooth muscle tissues Upper panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining)
Immunohistochemistry (IHC)
(Immunofluorescence staining of muscle tissue of 7 days old zebrafish embryo. Note reactivity in both striated and smooth muscle tissues Upper panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining)
Immunohistochemistry (IHC)
(Immunofluorescence staining of intestinal muscle tissue in 1 month old zebrafish embryo)
Immunohistochemistry (IHC)
(Immunofluorescence staining of intestinal muscle tissue in 1 month old zebrafish embryo)
Immunohistochemistry (IHC)
(Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo)
Immunohistochemistry (IHC)
(Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo)
Immunohistochemistry (IHC)
(Anti-Muscle Actin antibody IHC staining of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Anti-Muscle Actin antibody IHC staining of human colon, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Paraffin section of human cardiac muscle immunostained with HHF35 (1:250))
Immunohistochemistry (IHC)
(Paraffin section of human cardiac muscle immunostained with HHF35 (1:250))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
muscle actin
NCBI Official Symbol
LOC581500
NCBI Protein Information
muscle actin
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Product Notes
The IgG1 (Catalog #AAA249422) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Muscle Actin Antibody (clone HHF35) IHC-plus reacts with Chicken, Zebrafish, Pig, Rabbit, Rat, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Muscle Actin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB), ELISA (EIA), Electron Microscopy. IHC-Fr (1:200 - 1:1000) IHC-P (5 ug/ml) WB (1:1000 - 1:5000). Researchers should empirically determine the suitability of the IgG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Muscle Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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