Mouse MUC5AC (Mucin 5AC / Gastric Mucin) Monoclonal Antibody | anti-MUC5AC antibody
MUC5AC (Mucin 5AC / Gastric Mucin) Mouse Monoclonal Antibody
Gene Names
MUC5AC; TBM; leB; MUC5; mucin
Reactivity
Human, Monkey, Cow, Cat, Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
MUC5AC (Mucin 5AC / Gastric Mucin); Monoclonal Antibody; MUC5AC (Mucin 5AC / Gastric Mucin) Mouse Monoclonal Antibody; Apomucin Major Airway Glycoprotein; Mucin 5 subtype AC tracheobronchial; Mucin 5 Subtypes A And C; Mucin 5AC oligomeric mucus/gel forming; Tracheobronchial Mucin (TBM); anti-MUC5AC antibody
Host
Mouse
Reactivity
Human, Monkey, Cow, Cat, Mouse
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
2-11M1
Specificity
This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb 2-11M1 reacts with the protein backbone exclusively; it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then 2-11M1 will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
5654
Applicable Applications for anti-MUC5AC antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Frozen) (0.5-1ug/ml for 30 minutes at RT)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Frozen) (0.5-1ug/ml for 30 minutes at RT)
Cellular Localization
Cytoplasmic
Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Hu-Chromosome Location
11p15.5
Positive Control
MCF-7 cells or Stomach
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
References
Cancer Res.46: 3983-3989 (1986). Biochem. J. 254: 185-193 (1988). Int. J. Cancer 47: 304-310 (1991). J. Immunol. Methods 149: 105-113 (1992
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
>1; 000kDa
NCBI Official Full Name
mucin-5AC
NCBI Official Synonym Full Names
mucin 5AC, oligomeric mucus/gel-forming
NCBI Official Symbol
MUC5AC
NCBI Official Synonym Symbols
TBM; leB; MUC5; mucin
NCBI Protein Information
mucin-5AC
UniProt Protein Name
Mucin-5AC
Protein Family
UniProt Gene Name
MUC5AC
UniProt Synonym Gene Names
TBM
UniProt Entry Name
MUC5A_HUMAN
Uniprot Description
MUC5AC iso1:
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cytoplasm; extracellular space; fibril; Golgi lumen
Molecular Function: extracellular matrix structural constituent
Biological Process: fibril organization and biogenesis; O-glycan processing; phosphoinositide-mediated signaling
Research Articles on MUC5AC
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Product Notes
The MUC5AC muc5ac (Catalog #AAA439362) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MUC5AC (Mucin 5AC / Gastric Mucin) Mouse Monoclonal Antibody reacts with Human, Monkey, Cow, Cat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MUC5AC (Mucin 5AC / Gastric Mucin) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Frozen) (0.5-1ug/ml for 30 minutes at RT). Researchers should empirically determine the suitability of the MUC5AC muc5ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC5AC (Mucin 5AC / Gastric Mucin), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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