Human Mucin-5AC ELISA Kit | MUC5AC elisa kit
Human Mucin-5AC ELISA Kit
Gene Names
MUC5AC; TBM; leB; MUC5; mucin
Reactivity
Human
Synonyms
Mucin-5AC; Human Mucin-5AC ELISA Kit; MUC-5AC; Gastric mucin; Lewis B blood group antigen; LeB; Major airway glycoprotein; Mucin-5 subtype AC; tracheobronchial; Tracheobronchial mucin; TBM; MUC5AC; MUC5; MUC5AC elisa kit
Reactivity
Human
Specificity
Recombinant and natural Human MUC5A
Sequence Length
5654
Assay Type
Sandwich
Detection Range
0.78-50 ng/mL
Sensitivity
0.195 ng/mL
Intra-Assay CV
<=4.4%
Inter-Assay CV
<=7.1%
Recovery
95%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MUC5AC elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
585,570 Da
NCBI Official Full Name
mucin-5AC
NCBI Official Synonym Full Names
mucin 5AC, oligomeric mucus/gel-forming
NCBI Official Symbol
MUC5AC
NCBI Official Synonym Symbols
TBM; leB; MUC5; mucin
NCBI Protein Information
mucin-5AC
UniProt Protein Name
Mucin-5AC
Protein Family
UniProt Gene Name
MUC5AC
UniProt Synonym Gene Names
TBM
UniProt Entry Name
MUC5A_HUMAN
Uniprot Description
MUC5AC iso1:
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: cytoplasm; extracellular space; fibril; Golgi lumen
Molecular Function: extracellular matrix structural constituent
Biological Process: cellular protein metabolic process; fibril organization and biogenesis; O-glycan processing; phosphoinositide-mediated signaling; post-translational protein modification; protein amino acid O-linked glycosylation
