SDS-PAGE
(Lane 1: Mouse IgM, Non-Reduced. Lane 2: Mouse IgM, Reduced. Load: 1.0 ug per lane. Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.)
Mouse IgM Immunoglobulin | IgM immunoglobulin
Mouse IgM
Applications
User optimized
Purity
Mouse IgM was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.
Synonyms
Mouse IgM; Mouse Immunoglobulin M; IgM immunoglobulin
Purity/Purification
Mouse IgM was purified by delipidation, selective precipitation and tandem molecular sieve chromatography followed by dialysis.
Form/Format
Supplied as a liquid in 100mM Tris Chloride and 500mM Sodium Chloride, pH 8.0, with 0.1% Sodium Azide as a preservative.
Concentration
1.0 mg/ml (by UV absorbance at 280nm) (varies by lot)
Applicable Applications for IgM immunoglobulin
User optimized
Application Notes
A signle precipitin arc was observed against anti-Mouse IgM (mu chain specific), and anti-Mouse serum when assayed by Immunoelectrophoresis. No reaction was observed against anti-Mouse IgG F(c). Some ligh chain cross-reactivity will occur with anti-Mouse IgG.
Source Note
Prepared from normal mouse serum by a multi-step process
Species
Mouse
Biological Significance
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. It is the primary antibody against A and B antigens on red blood cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen.
Biohazard Information
This product is purified from non-human source material. However, it's recommended to observe full biohazard safety precautions when handling this material.
Preparation and Storage
Ships ambient or refrigerated. Upon receipt, store at 4 degree C while undiluted. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot and store at -20 degree C. Avoid freeze-thaw cycles.
SDS-PAGE
(Lane 1: Mouse IgM, Non-Reduced. Lane 2: Mouse IgM, Reduced. Load: 1.0 ug per lane. Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.)
SDS-PAGE
(Lane 1: Mouse IgM, Non-Reduced. Lane 2: Mouse IgM, Reduced. Load: 1.0 ug per lane. Predicted/Observed size - Non-Reduced: 900 kDa (Pentamer), 900 kDa (Molecule larger than can pass through gel), Reduced: 78 and 25 kDa, 75 and 25 kDa.)
Related Product Information for IgM immunoglobulin
Purified Mouse IgM
Product Categories/Family for IgM immunoglobulin
Similar Products
Product Notes
The IgM (Catalog #AAA537029) is an Immunoglobulin and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mouse IgM can be used in a range of immunoassay formats including, but not limited to, User optimized. A signle precipitin arc was observed against anti-Mouse IgM (mu chain specific), and anti-Mouse serum when assayed by Immunoelectrophoresis. No reaction was observed against anti-Mouse IgG F(c). Some ligh chain cross-reactivity will occur with anti-Mouse IgG. Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse IgM, Immunoglobulin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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